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Influence of Inorganic Nutrients on the activity of Enzyme, Nitrate reductase in the leaves of Mulberry, Morus alba (L) (M-5 variety)

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Author Affiliations

  • 1Indian Biodiesel Corporation, Baramati Tal- Baramati, Dist- Pune, INDIA
  • 2 Shardabai Pawar Mahila College, Shardanagar Tal – Baramati, Dist. Pune, INDIA

Res. J. Recent Sci., Volume 1, Issue (5), Pages 14-21, May,2 (2012)

Abstract

The protein content of mulberry leaves is directly related with the potential of nitrate reductase enzyme. Effect of kinetic parameters and inorganic mineral nutrients (Mg; Zn and Mo) on the velocity of nitrate reductase catalyzed biochemical reaction was studied using the leaves of mulberry, Morus alba (L) (M-5 variety). Maximum velocity (Vmax) was found registered for PH=7.4; temperature=32°C; incubation period= 30 minutes with vaccum infiltration manually at 5 minutes interval. For the purpose to determine Michaelis Menten constant (Km), the substrate concentration at which, the velocity of enzyme catalyzed biochemical reaction attain half of its maximum, attempt has been made towards the transformation of data on [S] and v. The key quotient: [(2v Vmax +S) ÷ v] – [S(1+Vmax) ÷ Vmax] was calculated. Plotting the key quotient verses the substrate concentration [S] has illuminated into a straight line intersecting both, X and Y axes at a point which correspond to : [(2Vmax2 + Km)÷Vmax]. The equation of the plot correspond to be derived as: Y = - [S] + [(2Vmax2+Km)÷ Vmax]. This plot is to be recognized as Punyamayee plot of enzyme kinetics. Accordingly the Michaelis Menten constant (Km) of nitrate reductase catalyzed biochemical reaction in assay sample of mulberry leaves was found elevated in assay sample of leaves of mulberry plant recipient of foliar spray of magnesium sulphate, Zinc sulphate and ammonium molybdate. The optimum dose for magnesium sulphate and Zinc sulphate was 2.5mM, while with the ammonium molybdate, it was 0.01mM. The enzyme nitrate reductase was found significantly influenced with the optimum dosage of inorganic nutrients like MgSO4; Zn and (NH4)2 Mo O4. The nitrate reductase activity may be considered as predictive test for protein rich yield of leaves in mulberry. Efficient use of inorganic nutrients for qualitative protein levels in mulberry leaves serve to orchestrate the moriculture practices and thereby the qualitative improvement in cocoon yield of silkworm, Bombyx mori(L).

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