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Genetic Manipulation of Gibberellin (GA) Oxidase Genes in Nicotiana sylvestris using constitutive promoter to modify Plant Architecture

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Author Affiliations

  • 1 Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK 2BBIO, Jai Research Foundation, Vapi, Gujarat, INDIA
  • 2

Res. J. Recent Sci., Volume 1, Issue (5), Pages 1-7, May,2 (2012)

Abstract

Gas is a large group of tetracyclic diterprenoid carboxylic acids. Gibberellins (GAs) control many aspects of plant development, including plant development, flowering, leaf expansion and growth. Leaf explants of Nicotiana sylvestris (Solanaceae) were used for Agrobacterium-mediated delivery of a range of GA-biosynthetic genes to determine the influence of their encoded enzymes on the production of bioactive GAs and plant stature in this species. Constructs were prepared containing the nptII gene for kanamycin resistance as a selectable marker, and the GA-biosynthetic genes, their expression under the control of the CaMV 35S promoter. The GA-biosynthetic genes comprised of PcGA2ox1 isolated from Phaseolus coccineus, and, is specific for C19-GAs and 2β-hydroxylates the bioactive GAs i.e. GA1 and GA4 and their immediate precursors GA20 and GA9, respectively. AtGA20ox1, isolated from Arabidopsis thaliana, the product from which catalyses the formation of C19-GAs, and MmGA3ox1 and MmGA3ox2, isolated from Marah macrocarpus, which encode functionally different GA 3-oxidases that convert C19-GAs to biologically active forms. Increase in stature was observed in plants transformed with AtGA20ox1, MmGA3ox1, MmGA3ox2 and MmGA3ox1 + MmGA3ox2, their presence and expression being confirmed by PCR and RT-PCR, respectively, accompanied by an increase in GA1 content, while PcGA2ox1 resulted in dwarf plant with four fold reduction of height and early flowering. The results are discussed in the context of regulating plant stature. Since this strategy would decrease the use of chemicals to promote plant growth and will result in value addition in ornamental industry, in an era of increasing demand, and ever changing consumer appetite.

References

  1. Bushman J.C.M. Globalisation – flower – flower bulbs – bulb flowers. Acta Horticulturae 673, 27-33 (2005)
  2. Spielmeyer W., Ellis M.H. andChandler P.M. Semidwarf (sd-1), green revolution rice, contains a defective gibberellin 20-oxidase gene, Proceedings Nat Acad Sci, USA 99, 9043–9048 (2002)
  3. Hedden P. and Kamiya Y., Gibberellin biosynthesis: enzymes, genes and their regulation, Plant Mol Biol 48, 431-460 (1997)
  4. Lange T. Molecular biology of gibberellin synthesis, Planta 204, 409-419 (1998)
  5. Phillips A.L. Genetic and transgenic approaches to improving crop performance. In: Plant Hormones: Biosynthesis, Signal Transduction, Action! (Davies PJ, ed) Kluwer Academic Publishers, Dordrecht, The Netherlands, 582-609 (2004)
  6. Rademacher R. Growth retardants: effects on gibberellin biosynthesis and other metabolic pathways, Ann Rev Plant Phy Mol Biol 51, 501-531 (2000)
  7. Rajapakse N.C., Young R.E., McMohon M.J. and Oi R. Plant height control by photoselective filters: current status and future prospects, Hort Tech 9, 616-624 (1999)
  8. Uzogara S.G., The impact of genetic modification of human foods in the 21st Century, Biotech Adv 18, 179-206 (2000)
  9. Mino M., Oka M., Tasaka Y. and Iwabuchi M. Molecular biology of the metabolism and signal transduction of gibberellins, and possible application to crop improvement. J Cr Impv 18, 365-390 (2006)
  10. Appleford N.E.J., Wilkinson M.D., Ma Q., Evans D.J., Stone M.C., Pearce P.S., Powers S.J., Thomas S.G., Jones H.D., Phillips A.L., Hedden P. and Lenton J.R., Decreased shoot stature and grain amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat, J Exp Bot 56, 112-120 (2008)
  11. Martin D.N., Proebsting W.M. and Hedden P. Mendel’s dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins. Proc of Nat Sci, USA, 94, 8907–8911 (1997)
  12. Itoh H., Ueguchi M.T., Kawaide H., Chen X., Kamiya Y. and Matsuoka M. The gene encoding tobacco gibberellin 3β-hydroxylase is expressed at the site of GA action during stem elongation and flower organ development, Plant J 20, 15-24 (1999)
  13. Yamaguchi S. Gibberellin biosynthesis in Arabidopsis, Phytochem Rev 5, 39-47 (2006)
  14. Hedden P. and Phillips A.L. Gibberellin metabolism: new insights revealed by the genes, Trends Plant Sci 5, 523-530 (2000)
  15. Mol J.N., Holton T.A. and Koes R.E. Floriculture: genetic engineering of commercial traits, Trends Biotech 13, 350-355 (1995)
  16. Newell C.A., Plant transformation technology, Mol Biotech 16, 53-65 (2000)
  17. Rani C.R., Reema C., Singh A. and Singh P.K., Salt tolerance of Sorghum bicolor cultivars during germination and seedling growth Res J Recent Sci., 1(3), 1-10 (2012)
  18. Bora A., Science Commmunication through Mass media Res J Recent Sci., 1(1), 10-15 (2012)
  19. Bhattacharya A., Ward D.A., Hedden A., Power J.B. and Davey M.R., Engineering gibberellin metabolism in Solanum nigrum L. by ectopic expression of gibberellin oxidase gene, Plant Cell Rep, 1214-8 (2012)
  20. Coles J.P., Phillips A.L., Croker S.J., Garcia-Lepe R., Lewis M.J. and Hedden P., Modification of gibberellin production and plant development in Arabidopsis by sense and antisense expression of gibberellin 20-oxidase genes, Plant J 17, 547-556 (1999)
  21. Sambrook J., Fritsch E. and Maniatis T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989)