Phytochemical Analysis and Antioxidant Properties of Lasianthera africana Leaves, Stems and Roots Extracts
- 1Department of Chemistry, Faculty of Science, Gombe State University, Gombe, Nigeria
- 2Department of Chemistry, Faculty of Science, University of Ibadan, Ibadan, Nigeria
- 3Phytomedicine Research Centre, Botany Department, University of Fort Hare, Alice, South Africa
Res.J.chem.sci., Volume 6, Issue (9), Pages 19-26, September,18 (2016)
The study evaluated the phenolic content and antioxidant capacities of Lasianthera africana leave stem and root extractswhich is utilized for the management of oxidative stress related ailments in eastern Nigeria. The plant’s antioxidant and free radical scavenging properties was evaluated in vitro against ferric reducing agent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), hydrogen peroxide (H2O2) and 2,2-azinobis (3-ethylbenzothiazolie-6-sulphonic acid) diammonium salt (ABTS). The total phenolic content and the total flavonoids of the extracts were determined and their related effect on the antioxidant activity was evaluated. The reducing capacity of the standard drug, butylated hydroxytoluene (BHT) was found to be lower than those of the extracts. The free radical quenching effect of the extracts against DPPH, NO, H2O2 and ABTS radicals were concentration dependent with IC50 of 0.30 ,0.29, 0.32 and 0.30 mg/mL for the leaves; 0.31,0.29,0.31 and 0.31 mg/mL for the stem; and 0.30, 0.27, 0.31 & 0.30 mg/mL for the roots respectively. The total phenolic content of the extracts were 18.21±0.1, 16.67±0.5 and 19.5±0.3mg GAE/g DW while the total flavonoids were 6.39±0.2, 6.83±0.1, 5.15±0.2mg QE/g DW for the leaves, stem and root extract respectively. Our results showed that Lasianthera africana ethanol extracts are good sources of free radical scavengers and therefore could be used in the management of oxidative disorders.
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