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Development of Electrochemical Biosensor for the Detection of Klebsiella pneumoniae as Biological Weapon

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Author Affiliations

  • 1Material Science and Electrochemistry Lab., Dept. of Chemistry, Ch. Devi Lal University, Sirsa, Haryana, 125 055, INDIA

Res.J.chem.sci., Volume 5, Issue (12), Pages 88-97, December,18 (2015)

Abstract

Rapid, explicit detection and identification of Biological Warfare Agents (BWAs) with early warning signals for detecting possible biological attack is a main challenge for health, military and other government defence agencies. The current research is focussed on development of electrochemical biosensors for the detection of biological warfare agent. For this purpose, a carbon based (Graphene) working electrode containing enzyme alkaline phosphatase, cellulose acetate and Poly Vinyl Pyrolidone (PVP), Ferrocene, Horseradish peroxidase, aq. KOH was fabricated. It is then combined with Ag/AgCl reference and a platinum electrode (auxiliary) to form a three electrode based electrochemical biosensor for the electrochemical detection of Klebsiella pneumoniae as biological warfare agent in the presence and absence of Fe3O4 nanoparticles. Fe3O4 nanoparticles were produced by sol-gel technique and were characterized by UV-visible, FTIR, TEM and XRD techniques. Change in current response and OCP values helps in the detection of biological warfare agent in presence and absence of Fe3O4 nanoparticles. Effects of temperature, stirring and Fe3O4 nanoparticles on the BWA have also been investigated.

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