International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Preliminary optimization of PHB production by Vibrio sp. MCCB 237 isolated from Marine Environment

Author Affiliations

  • 1School of Environmental Studies, Cochin University of Science and Technology, Kochi, INDIA

Res.J.chem.sci., Volume 4, Issue (5), Pages 10-13, May,18 (2014)


Polyhydroxybutyrate (PHB) is the most widely studied polyester of microbial origin and is the representative of a group of such polyesters known as polyhydroxyalkanoates (PHA). PHAs are polymers that are synthesized by microorganisms under imbalanced growth conditions and serve as carbon and energy reserve. The material properties of PHAs are similar to various petrochemically-derived thermoplastics and elastomers that have immense use in our daily life and hence are considered as possible substitutes for synthetic, non-degradable polymers. The commercial exploitation of these polymers is still restricted by the high cost of production incurred. The success in the biodegradable plastic strategy largely depends on the isolation of potent PHB-producing bacteria and optimizing culture parameters for maximum PHB biosynthesis. In the present study the efficacy of various carbon and nitrogen sources were investigated for enhanced PHB production using Vibrio sp. MCCB 237 isolated from marine environment, which provides a meagerly exploited resource for possible biopolymer-producing novel bacteria. Quantification of PHB was done spectrophotometrically and characterized using FTIR. Among the tested carbon sources pectin followed by glycerol gave the highest yields and among the nitrogen sources, yeast extract yielded better quantity of PHB. These results are being implemented in further optimization of culture parameters.


  1. Lemoigne M., Products of dehydration and polymerization of hydroxybutyric acid, Bull Soc Chem Biol., 770-782 (1926)
  2. Lee S.Y., Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria, Trends Biotechnol., 14, 431-438 (1996)
  3. Anderson A.J. and Dawes E.A., Occurrence, Metabolism, Metabolic Role, and Industrial Uses of Bacterial Polyhydroxyalkanoates, Microb. Rev., 54(4), 450-472 (1990)
  4. Wang J. and Yu H., Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures, Appl Microbiol Biotechnol., 75, 871-878 (2007)
  5. Madison L.L. and Huisman G.W., Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic, Microbiol Mol Biol R., 63(1), 21-53 (1999)
  6. Patnaik P.R., Perspectives in the Modeling and Optimization of PHB Production by Pure and Mixed Cultures, Crit Rev Biotechnol., 25, 153-171 (2005)
  7. Grothe E., Moo-Young M. and Chisti Y., Fermentation optimization for the production of poly(-hydroxybutyric acid) microbial thermoplastic, Enzyme Microb Technol., 25, 132-141 (1999)
  8. Grothe E. and Chisti Y., Poly(-hydroxybutyric acid) thermoplastic production by Alcaligenes latus: behavior of fed-batch cultures, Bioprocess Eng., 22, 441-449 (2000)
  9. Tamer I.M., Moo-Young M. and Chisti Y., Optimization of poly(-hydroxybutyric acid) recovery from Alcaligenes latus: combined mechanical and chemical treatments, Bioprocess Eng., 19, 459-468 (1998)
  10. Silva G.P., Mack M. and Contiero J., Glycerol: A promising and abundant carbon source for industrial microbiology, Biotechnol Adv., 27, 30-39 (2009)
  11. Zhao L., Fan F., Wang P. and Jiang X., Culture medium optimization of a new bacterial extracellular polysaccharide with excellent moisture retention activity, Appl Microbiol Biotechnol., 97, 2841-2850 (2013)
  12. Aldor I.S. and Keasling J.D., Process design for microbial plastic factories: metabolic engineering of polyhydroxyalkanoates, Curr. Opinion Biotech., 14, 475-483 (2003)
  13. Zobell C.E., Marine Microbiology, Chronican Botanica Co., Waltham Mass, 240 (1946)
  14. Ostle A.G. and Holt J.G., Nile Blue A as a Fluorescent Stain for Poly--Hydroxybutyrate, Appl Environ Microb.44(1), 238-241 (1982)
  15. Law J.H. and Slepecky R.A., Assay of Poly-hydroxybutyric acid, J Bacteriol., 82, 33-36 (1961)
  16. Locatelli G., Silva G.D., Finkler L. and Finkler C.L.L., Acid Hydrolysis of Pectin for Cell Growth of Cupriavidus necator, Biotech., 11(1), 29-36 (2012)
  17. Sundar Raj A.A., Rubila S., Jayabalan R., Ranganathan T.V. A Review on Pectin: Chemistry due to general properties of pectin and its pharmaceutical uses, 1:550 doi 10.4172/scientificreports.550 (2012)
  18. Wang Z.X., Zhuge J., Fang H. and Prior B.A., Glycerol production by microbial fermentation: A review, Biotechnol Adv., 19(3), 201203 (2001)
  19. Ashby R.D., Solaiman D.K.Y. and Foglia T.A., Bacterial poly(hydroxyalkanoate) polymer production from the biodiesel co-product stream, J Polym Environ., 12, 105-112 (2004)
  20. Bormann E.J. and Roth M., The production of polyhydroxybutyrate by Methylobacterium rhodesianumand Ralstonia eutropha in media containing glycerol and casein hydrolysates, Biotechnol Lett., 21, 1059-1063 (1999)
  21. Valappil S.P., Misra S.K., Boccaccini A.R., Keshavarz T., Bucke C. and Roy I., Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterized Bacillus cereus SPV, J. Biotechnol., doi:10.1016/j.jbiotec.2007.03.013 (2007)