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Biological Production of Xylitol from Corn Husk and Switchgrass by Pichia stiptis

Author Affiliations

  • 1Department of Chemical Engineering, Indian Institute of Technology Roorkee, Roorkee, INDIA

Res.J.chem.sci., Volume 3, Issue (3), Pages 58-64, March,18 (2013)

Abstract

Xylitol is a naturally occurring sugar substitute and widely used sweetener. In the present study, xylitol was produced through biological reduction pathway using yeast strain Pichia stiptis CBS 5773 by extracting xylose from agricultural residue like corn husk and switchgrass hemicelluloses as substrate. Acid treatment with sulphuric acid followed by detoxification with 2% Ca(OH) was done to reduce the inhibiting factors like acetic, furfuryl and tannic acids and phenolic compounds. A comparative study of xylitol production using corn husk, switchgrass, their mixture (corn husk and switchgrass) and pure xylose was done. Yield of xylitol after 72 h of fermentation at 32°C and pH 5.7 using corn husk, switchgrass, their mixture and pure xylose was found to be 0.62 g, 0.48 g, 0.59 g and 0.73 g respectively per g of xylose initially present. Xylose consumption efficiency was 63.4%, 52%, 60% and 73% in corn husk, switchgrass, their mixture and pure xylose hydrolysates respectively under optimal condition. Therefore production was efficient using pure xylose and corn husk in comparison to switchgrass and mixture of corn husk and switchgrass under optimal condition.

References

  1. Uhari M., Kontiokari T. and Niemela M., A Novel Use of Xylitol Sugar in Preventing Acute Otitis Media, Pediatrics,879–884 (1998)
  2. Hyvonen L. and Slotte M., Alternative Sweetening of yoghurt, J. Food Technol., 97-112 (1983)
  3. Leathers, T.D., Bioconversion of maize residues to value-added coproducts using yeast like fungi, FEMS Yeast Res., 133–140 (2003)
  4. Rao R.S., Jyothi C.P., Prakasham R.S., et al., Xylitol production from corn fiber and sugarcane bagasse hydrolysatess by Candida tropicalis, Bioresource Technol., 1974–1978 (2006)
  5. Roberto I.C., Felipe M.G.A., Lacis L.S., et al., Utilization of Sugar Cane Bagasse Hemicellulosic Hydrolyzate by Candida guilliermondii for Xylitol Production, Bioresource Technol, 271-275 (1991)
  6. Roberto I.C., Mancilha I.M., Souza C.A. de, et al., Evaluation Of Rice Straw hemicellulose hydrolysates in the production of xylitol by Candida guilliermondii,Biotechnol. Lett., 1211-1216 (1994)
  7. Converti A., Perego P. and Domínguez J. M., Xylitol Production from Hardwood Hemicellulose Hydrolysates by Pachysolen tannophilus, Debaryomyces hansenii and Candida guilliermondii.Appl. Biochem. Biotech.,141-151(1999)
  8. Cruz M. J., Dominguez J. M., Dominguez H., et al., Xylitol production from barley bran hydrolysates by continuous fermentation with Debaromyces hansenii,Biotechnol. Lett., 1895–1898 (2000)
  9. Chiang C. and Knight S.G., Metabolism of D-xylose by moulds, Nature,79-81 (1961)
  10. Barbosa M.F.S., Medeiros M.B. de, Mancilha I.M. de, et al., Screening of yeasts for production of xylitol from D-xylose and some factors which affect xylitol yield in Candida guilliermondii, J. Indust. Microbiol., 241-251 (1988)
  11. Yoshitake J., Shimamura M. and Imai T., Xylitol production by Corynebacterium species, Agric. Biol Chem., 2251-2259 (1973)
  12. Izumori K. and Tuzaki K., Production of xylitol from D-xylose by Mycobacterium smegmatis, J. Ferment. Technol., 33-36 (1988)
  13. Parajo J.C., Dominguez H. and Dominguez J.M., Biotechnological production of xylitol Part1: Interest of xylitol and fundamentals of its biosynthesis, Bioresource Technol.,191-201 (1998)
  14. Mussatto S.I. and Roberto I.C., Alternatives for detoxification of diluted acid lignocellulosic hydrolysates for use in fermentative processes: a review, Bioresource Technol., 1-10 (2004)
  15. Olsson L. and Hagerdal-Hahn B., Fermentation of lignocellulosic hydrolyzates for ethanol production, Enzyme Microb. Technol., 312–331 (1996)
  16. Vallander L. and Erickson K.E.L., Production of ethanol from lignocellulosic materials: State of the art, Adv. Biochem. Eng., 63–95 (1990)
  17. Tsao G.T., Cao N. and Gong C.S., Hemicellulose conversion, Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseperation, 1391–1400 (1999)
  18. Pandey A, Soccol C. R., Nigam P., et al., Biotechnological potential of agro-industrial residues. I. Sugarcane bagasse, Bioresour. Technol., 69–80 (2000)
  19. Mancilha I.M. and Karim M.N., Evaluation of ion exchange resins for removal of inhibitory compounds from corn husk hydrolysates for xylitol fermentation, Biotechnol. Prog.,1837–1841 (2003)
  20. Dien B.S., Nichols N.N., Bryan P.J.O’, et al., Development of new ethanologenic Escherichia coli strains for fermentation of lignocellulosic biomass, Appl. Biochem. Biotechnol., 181–196 (2000)
  21. Cassland P. and Jonsson L.J., Characterization of a gene encoding trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature, Appl. Microbial biotechnol., 393–400 (1999)
  22. Dien B.S., Hespell R.B., Ingram L.O. et al., Conversion of corn milling fibrous co-products into ethanol by recombinant Escherichia coli strains K011 and SL40, World J. Microbiol. Biotechnol., 619–625 (1997)
  23. Rivas B., Torre P., Domfinguez J.M. et al., Carbon material and bioenergestic balances of xylitol production from corncob by Debaryomyces hansenii,Biotechnol. Prog., 706–713 (2003)
  24. Silva S.S., Maria G.A., Silva B.A. et al., Acid hydrolysis of Eucalyptus grandischips for microbial production of xylitol, Process Biochem., 63–67 (1998)
  25. Kadam K.L. and Mcmillan J.D., Availability of corn husk as a sustainable feedstock for bioethanol production, Bioresour. Technol., 17–25 (2003)
  26. Torget R., Walter P., Grohmann H.M.K., Dilute acid pretreatment of corn residues and short-rotation woody crops, Appl. Biochem. Biotech., 75–86 (1991)
  27. Updegraff D.M., Semi-micro determination of cellulose in biological materials, Anal. Biochem., 420–424 (1969)
  28. Roe J.H. and Rice E.W., A photometric method for the determination of free pentoses in animal tissues, J. Biol. Chem., 507-512 (1948)
  29. Yahashi Y., Horitsu H., Kawai K. et al., Production of xylitol from -xylose by Candida tropicalis: the effect of -glucose feeding, Journal of Ferment. Bioeng., 148–152 (1996)
  30. Alves L.A., Felipe M.G.A., Almeida J.B., Pretreatment of Sugarcane Bagasse Hemicellulose Hydrolysates for Xylitol Production by Candida guilliermondii, Appl. Biochem. Biotechnol., 89-98 (1998)