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Enzyme Biosensor Electrode Based on Immobilized Urease-Alginate: Preparation, Characterization and Significance

Author Affiliations

  • 1Department of Chemistry, Mahila MahaVidyalaya, Banaras Hindu University, Varanasi-221005, INDIA

Res.J.chem.sci., Volume 3, Issue (10), Pages 69-72, October,18 (2013)


Pigeonpea urease was immobilized on calcium alginate beads. The maximum immobilization, 50.5% was observed. The undertaken work compares the kinetic properties of soluble urease with the immobilized enzyme. The immobilized urease detected a shift in its optimum pH from 7.5 to 7.0 in Tris/acetate buffer. The optimim temperature also shifted from 47 to 67 degrees compared with the soluble enzyme. Alginate-immobilized pigeonpea urease had a higher Km (10.5 mM) than that of the soluble enzyme (7.3 mM). An attempt has been made to use immobilized beads to prepare a urea biosensor developed from potentiometric pH glass electrode coupled to a calomel electrode. Furthermore, in a pilot study a calibration plot was made for estimation of serum urea using the enzyme biosensor electrode.


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