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A Calorimetric Investigation of Chromium Interaction with Jack bean Urease

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Author Affiliations

  • 1Chemistry Department, faculty of science, Islamic Azad University, Takestan Branch, Takestan, IRAN
  • 2Chemistry Department, Payame Noor University (PNU), Abhar, IRAN
  • 3Institute of Biochemistry and Biophysics, University of Tehran, Tehran, IRAN

Res.J.chem.sci., Volume 2, Issue (5), Pages 85-88, May,18 (2012)

Abstract

Urease activity is often used for characterization of microbial viability in soil. The aim of the investigation was to measure the influence of chromium (III) on urease activity. Urease activity in pure solution was so sensitive for Cr (III), which caused inhibition of urease activity significantly. The complexation between Cr3+and Jack bean urease is examined using isothermal titration calorimetry (ITC). It was found that chromium ions acted as a noncooperative inhibitor of JBU, and there is a set of 12 identical and independent binding sites for Cr3+ ions. The association equilibrium constant is 6.79×106 L-1.mol, indicating the strong interaction of Cr3+ ion with JBU. The molar enthalpy of binding is DH = 15.10 kJmol-1.

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