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Spectrophotometric Determination of Phenol in Micellar Medium

Author Affiliations

  • 1Department of Chemistry, Govt. V.Y.T. Autonomous College Durg, Chhattisgarh, INDIA

Res.J.chem.sci., Volume 2, Issue (12), Pages 6-10, December,18 (2012)


A simple and sensitive spectrophotometeric method for the determination of Phenol based on its oxidation by N-bromophthalimide (NBP) is proposed. The resulting bromoderivative of phenol oxidizes leucocrystal violet to crystal violet in aqueous medium, the absorbance of which is measured at 595 nm. The colour intensifies with the addition of surfactant, cetyltrimethyl ammonium bromide (CTAB). Beer’s law is obeyed in the range of 0.02 – 0.22 µg mL-1 of phenol. The molar absorbtivity of the dye is found to be 3.13 x 105 mol-1 cm-1 and sandell’s sensitivity as calculated from the slope is found to be 3x10-4 µg cm-2The method has been successfully applied for the determination of phenol in coke oven effluents and the result obtained, compares well with other reported methods.


  1. James J. Schauer, Michael J. Kleeman, Glen R.C., Bernd R.T. Simoneit, Environ. Sci. Technol.,35, 1716-1728 ( 2001)
  2. James W. Moore, Ramamoorthy S., Organic Chemicals in Natural Waters: Applied Monitoring and Impact Assessment, (Springer Verlag NewYork), (1984)
  3. Marcelo F. Pistonesi, María Susana Di Nezio, María Eugenia Centurión, Miriam E. Palomeque, Adriana G. Lista, Beatriz S. Fernández, Determination of phenol, resorcinol and hydroquinone in air samples by synchronous fluorescence using partial least-squares (PLS), Talanta, 69(5), 1265-1268 (2006)
  4. DeSelms R.H., UV-Active Phenol Ester Compounds,(Enigen Science Publishing Washington DC), (2008)
  5. Svobodová A, Psotová J, Walterová D., Natural phenolics in the prevention of UV – induced skin damage, A review. Biomed papers, 147, 137 (2003)
  6. Agency for toxic substances and disease registry (ATSDR),Toxicological profile for phenol (Draft for public comment), (US department of public health and human services, public health service, Atlanta, GA) (2006)
  7. Marcos Grünhut, Miriam E. Palomeque, Adriana G. Lista, Beatriz S. Fernández, Enzymatic reverse FIA method for total phenols determination in urine samples, Talanta, 71, 1520 (2007)
  8. Renato Baciocchi, Marina Attinŕ, Giusy Lombardi, Maria Rosaria Boni, Fast determination of phenols in contaminated soils, Journal of Chromatography A,911, 135-141(2001)
  9. Wei Cao, Xuemin Mu, Jinghe Yang, Wenbo Shi, Yongcun Zheng, Flow injection–chemiluminescence determination of phenol using potassium permanganate and formaldehyde system, Spectrochimica Acta A,66, 58 (2007)
  10. Qing Xiang Zhou, Jun Ping Xiao, Cun Ling Ye, Xin Ming Wang, Enhancement of Sensitivity for Determination of Phenols in Environmental Water Samples by Single-drop Liquid Phase Microextraction Using Ionic Liquid prior to HPLC, Chinese Chemical Letters, 17, 1073 (2006)
  11. Lida Fotouhi, Mahsa Ganjavi and Davood Nematollah, Electrochemical Study of Iodide in the Presence of Phenol and o-Cresol: Application to the Catalytic Determination of Phenol and o-Cresol, Sensors,4, 170-180 (2004)
  12. Fiamegos Y.C., Stalikas C.D., Pilidis G.A., Karayannis M.I., Synthesis and analytical applications of 4-aminopyrazolone derivatives as chromogenic agents for the spectrophotometric determination of phenols, Analytica Chimica Acta, 403, 315 (2000)
  13. Sally N. Jabrou, Extraction of Phenol from Industrial Water Using Different Solvents, Res. J. Chem. Sci.,2(4), 1-12(2012)
  14. Pawar M.J., Nimbalkar V.B., Res. J. Chem. Sci., Synthesis and phenol degradation activity of Zn and Cr doped TiO2 Nanoparticles, 2(1), 32-37 (2012)
  15. Vedula Ravi Kiran, Balomajumder Chandrajit, Simultaneous Adsorptive Removal of Cyanide and Phenol from Industrial Wastewater: Optimization of Process Parameters, Res. J. Chem. Sci., 1(4), 30-39 (2011)
  16. Karim D. Khalaf, Berween A. Hasan, Angel Morales-Rubio, Miguel de la Guardia, Spectrophotometric determination of phenol and resorcinol by reaction with p-aminophenol, Talanta, 41, 547 (1994)
  17. Fiamegos Y., Stalikas C., Pilidis G., 4-Aminoantipyrine spectrophotometric method of phenol analysis: Study of the reaction products via liquid chromatography with diode-array and mass spectrometric detection, Analytica Chimica Acta, 467, 105 (2002)
  18. Shukla A., Sharma S., Shrivas K., Patel K.S., Peter Hoffman, Determination of Phenol in Wastewater, Chem.Anal.(Warsaw),50, 905 (2005)
  19. Chunli Kang, Ying Wang, Runbo Li, Yaoguo Du, Jun Li, Bowen Zhang, Liming Zhou, Yuzhong Du , Modified spectrophotometric method for the determination of trace amounts of phenol in water, Microchemical Journal, 64, 161(2000)
  20. Djerass C., Barkot M., Chem Rev.,46,147 (1960)
  21. Bailar J.C., The Chemistry of Co-ordination compounds, (Reinhold, NewYork), 14 (1956)
  22. Agrawal O., Gupta V.K., Sub-Parts-per-Million Spectrophotometric Determination of Phenol and Related Pesticides Using Diazotized p-Aminoacetophenone, Microchemical Journal., 62, 147 (1999)
  23. Lacoste R.J., Venable S.H., Stone J.C., Modified 4-aminoantipyrine colorimetric method for phenols, Anal. Chem., 31, 1246 (1959)
  24. Yurchenko V.V., Depovskir N.S., Zulfigasov O.S., Philipenta, Zh A.T., Anal Khim.92, 2033(1987)Chem Abstr.,10562a. (1988)
  25. Ramachandran K.N., Gupta V.K., A new method for photometric determination phenol, Chem Anal., 37 , 489 (1992)
  26. Amlathe S., Upadhyaya S., Gupta V.K., Spectrophotometric determination of trace amounts of phenol in waste water and biological fluids, Analyst, 112, 1463 (1987)