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A study of binding of DNA extracted from onion with Ruthunium(II) Polypyridyl Complexes

Author Affiliations

  • 1Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil, Tamilnadu, India
  • 2Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil, Tamilnadu, India
  • 3Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil, Tamilnadu, India

Res.J.chem.sci., Volume 12, Issue (3), Pages 20-24, October,18 (2022)


In an aqueous solution, the binding of the Ru(II) polypyridyl complex [RuL3]2+ (where L=bpy, dmbpy) with onion DNA has been investigated using absorption and emission spectrum methods. The MLCT absorption maxima for both compounds are in the 445–460nm region. Using data on absorption intensity and emission investigations, the binding constant (Kb) for these processes is calculated from the Benesi-Hildebrand equation. The luminophore and DNA molecule interact at the ground state in a hydrophobic way. These complexes bind to DNA through intercalative mode. The Kb value depends on the nature of the DDNA and also on the ligand used.


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