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Quantitative estimation of vinca alkaloid in Catharanthus roseus under stress conditions

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Author Affiliations

  • 1Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Faridabad, Haryana India, and Department of Chemistry, Gargi, College New Delhi, India
  • 2Department of Biotechnology, Manav Rachna International Institute of Research and Studies, Faridabad, Haryana India, and Department of Chemistry, Gargi, College New Delhi, India

Res.J.chem.sci., Volume 12, Issue (3), Pages 15-19, October,18 (2022)

Abstract

Vinca alkaloids are anti-mitotic and anti-microtubule alkaloids derived from the periwinkle plant, Catharanthus roseus. Vinca alkaloids are utilized in the treatment of cancer. They are a type of cytotoxic medication that functions by preventing cancer cell division during the cell cycle. They work on tubulin to prevent it from forming microtubules, which are essential for cellular division. As a result, they prevent beta-tubulin polymerization in dividing cells. Vinca alkaloids are the second most frequently utilized family of cancer medications. For therapeutic purposes, vinca alkaloids are commonly used in combination with chemotherapies. Periwinkle plants produce vinca alkaloids naturally in their leaves, but the concentration of these alkaloids is quite low within the plant. The expense of extracting and isolating these alkaloids from vinca plants has been expensive throughout. Vinca alkaloids are secondary metabolites and their synthesis can be increased in response to stress and unfavorable environmental conditions. Synthetic vinca alkaloids have been produced to fight cancer and immunological suppression, however, it is still under research and is not extensively used. The purpose of this study is to examine if vinca alkaloids concentrations increase when plants are subjected to binary stress. We have extracted the phytochemical, vinca alkaloid from Catharanthus roseus by cultivating it under different controlled conditions to inspect the concentration of the phytochemical produced. Further, we have identified the stress circumstances that may result in the enhanced production of vinca alkaloid. Thereafter we have analyzed the extracts of vinca alkaloid via qualitative and quantitative examination.

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