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Determination of Hexazinone in Environmental Samples by Uv-Vis Spectrophotometry-Partial Least Squares Regression

Author Affiliations

  • 1Institute of Biotechnology, University of Papaloapan, CP 68301, Tuxtepec, Oaxaca, MEXICO
  • 2Environmental Engineering, Universidad del Mar, CP 70902, Puerto Ángel, Oaxaca, MEXICO

Res.J.chem.sci., Volume 1, Issue (8), Pages 36-41, November,18 (2011)


A simple and reliable analytical method is proposed for the spectrophotometric determination of hexazinone in water and soil leachates, where spectral interference from organic matter is avoided by means of a chemometric tool. Binary mixtures containing from 0.5 to 14 µg mL-1 of the herbicide and from 0 to 30 µg mL-1 of sodium salt of humic acids were prepared for the calibration set. The limit of detection was of 0.1 and the limit of quantification of 0.4 µg mL-1; a precision of 2.0 % was estimated, expressed as a relative standard deviation in percentage. Satisfactory mean recoveries ± confidence limits were obtained in synthetic mixtures (102 ± 2 %), as well as in tap water (102 ± 1 %), well water (103.8 ± 0.3 %) and soil leachates (96 ± 5 %). Unfortunately, a smaller mean recovery was found for sea water (66 ± 15 %), probably due to the high salinity of the matrix reduces the solubility of hexazinone.


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