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Effect of Deltamethrin and a Neem Based Pesticide Achook on Some Biochemical Parameters in Tissues Liver, Ovary and Muscle of Zebrafish Danio rerio (Cyprinidae)

Author Affiliations

  • 1Zebrafish Laboratory Department of Zoology, DDU Gorakhpur University, Gorakhpur (UP) INDIA

Res.J.chem.sci., Volume 1, Issue (4), Pages 125-134, July,18 (2011)


The present study is aimed to evaluate the changes in total protein, total free amino acid content and nucleic acids (DNA and RNA) in the liver, ovary and muscle of Zebrafish after exposure to 96 h LC, LC10 and LC20 of Deltamethrin and neem based pesticide Achook. It was found that the protein content was reduced to 45, 68, 65% (after Deltamethrin exposure) and 54, 81, 85% (after Achook exposure) as compared to the controls (100%) after 16 days in the liver, ovary and muscle, respectively at LC20 exposure. The total free amino acid contents in liver were enhanced to 146 and 142% of controls (100%) after exposure to LC20 of Deltamethrin and Achook respectively.However,in the ovary of treated fishes the total free amino acid content was significantly (P<0.05) reduced. In addition, DNA content was reduced to 26, 40 and 38% of controls (100%) in liver, ovary and muscle respectively after 16 days exposure to LC20 of Deltamethrin. The reduction in DNA content from the control was 37, 46 and 44% in liver, ovary and muscle respectively due to Achook at the same concentration and exposure period as that of Deltamethrin. Similarly, RNA contents were also reduced in the liver, ovary and muscle significantly after Deltamethrin and Achook exposure. It was observed that all the changes were concentration as well as time dependent. This study will reflect the potential role of these biochemical parameters for assessment of aquatic pollution as far as the natural pesticides are concerned.


  1. Rand G.M., Fundamentals of aquatic toxicology. Taylor and Francis Publishing, Washington, D.C., 1150 (1995)
  2. Bradbury S.P. and Coats J.R., Toxicological and toxicodynamics of pyrethroid insecticides, Environ. Toxicol. Chem.,8, 373-386 (1989)
  3. Yildirim M.Z., Benli K.A.C., Benli M., Ozukul A., Erkoc F. and Oner K., Acute toxicity, behavioural changes and histopathological effects of Deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (Oreochromis niloticus L.) fingerlings, Environ. Toxicol.,21, 614-620 (2006)
  4. Harikrishnan R., Rani M.N. and Balasundaram C., Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophilainfection, Aquacult.,221, 41-50 (2003)
  5. Morgan E.D., Azadirachtin, a scientific gold mine, Bioorganic and Medicinal Chem., 17,4096-4105 (2008)
  6. Winkaler E.U., Santos T.R.M., Machado-Neto J.G. and Martinez C.B.R., Acute lethal and sub-lethal effects of neem leaf extract on the Neotropical freshwater fish, Prochilodus lineatus, Comp. Biochem. Physiol., Part C,145, 236-244 (2007)
  7. Sharma D.K. and Ansari B.A., Effect of the synthetic Pyrethroid Deltamethrin and the neem based pesticide Achook on the reproductive ability of Zebrafish, Danio rerio(Cyprinidae), Arch. Pol. Fish.,18, 157-161 (2010)
  8. Singh P.K. and Ansari B.A., Effect of Neem based formulations (Nimbecidine and Ultineem) on the spawning success of Zebrafish Danio rerio (Cyprinidae), The Bioscan,5(4), 669-672 (2010)
  9. Ahmad M.K. and Ansari B.A., Toxicity of Neem based pesticide Azacel to the embryo and fingerlings of Zebrafish, Danio rerio(Cyprinidae), World J. Zool.,6(1), 47-51 (2011)
  10. Ansari S. and Ansari B.A., Embryo and fingerling toxicity of Dimethoate and effect on fecundity, viability and survival of Zebrafish, Danio rerio (Cyprinidae), World J. Fish Marine Sci.,3(2), 167-173 (2011)
  11. Regoli F. and Principato G., Glutathione dependent and antioxidant enzymes in mussel, Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: Implications for the use of biochemical biomarkers, Aquat. Toxicol.,31, 143-164(1995)
  12. Ramesh M., Toxicity of copper sulphate on some haematological parameters of a fresh water teleost Cyprinus carpio var. communis, J. Ind. Fisheries Association,28, 131-136 (2001)
  13. O.E.C.D., Guidelines for Testing of Chemicals, Guideline 210 “Fish, Early-life Stage Toxicity Test.” Adopted July 17 (1992)
  14. Ansari B.A. and Kumar K., A record of Zebra danio (Brachydaniorerio) (Cyprinidae) from Uttar Pradesh with notes on sexual dimorphism, J. Adv. Zool.,3(1), 88-89 (1982)
  15. Schneider W.C., Determination of nucleic acids in tissues by pentose analysis. In: Colowick, S.P. and N.O. Kaplan (Ed.) Methods in Enzymology, Academic Press, New York, 680-684 (1957)
  16. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurements with Folin-phenol reagent, J. Biol. Chem.,193, 265-275 (1951)
  17. Spies J.R., Colorimetric procedures for amino acids. In: Colowick, S.P. and N.O. Kaplan, (Ed.) Methods in Enzymology, Academic Press, New York, 464-471 (1957)
  18. Anees M.A., Acute toxicity of four organophosphorus insecticides to a fresh water teleost, Channa punctatus (Bloch), Pak. J. Zool.,7, 135-141 (1975)
  19. Kumar K. and Ansari B.A., Malathion Toxicity: Skeletal Deformities in Zebrafish Brachydaniorerio, Cyprinidae), Pestic. Sci.,15, 107-111 (1984)
  20. W.H.O., Deltamethrin, environmental health criteria 97. World Health Organization, Geneva, 133 (1990)
  21. Kumar S., Lata S. and Gopal K., Deltamethrin induced physiological changes in freshwater catfish, Heteropneustes fossilis, Bull. Environ. Contam. Toxicol.,62, 254-258 (1999)
  22. Ogbuewu I.P., Uchegbu M.C., Okoli I.C. and Iloeje M.U., Toxicological effects of leaf meal of ethnomedicinal plant –Neemon serum biochemistry of crossbred New Zealand white typed rabbit bucks, Report and Openion,2, 54-57 (2010)
  23. Dutta P., Bhattacharyya P.R., Rabha D.N., Bordoloi N.C., Chowdhury P.K., Sharma R.P. and Barua J.N., Feeding deterrents for Philosamia ricini (Samia cynthia Sub-sp. Ricini) from Tithonia deversifolia, Phytoparasit.,14(1), 77-80 (1986)
  24. Hussein S.Y., El-Nasser M.A. and Ahmed S.M., Comparative studies on the effects of herbicides Atrazine on freshwater fish, Oreochromis niloticus and Chrysichthyes auratus at Assuit, Egypt, Bull. Environ. Contam. Toxicol., 57, 503-510 (1996)
  25. Swietla A. and Zuk J., Cytotoxic effects of maleic hydrazine, Mutation Res. /Rev. in Genetic Toxicol.,55(1), 15-30 (1978)
  26. Katherine N.P., Bjorn T.B. and Stephen D.M., Effects of hexaxinone and atrazine on the physiology and endocrinology of smolt development in Atlantic salmon, Aquat. Toxicol.,84(1), 27-37 (2007)
  27. Anita Susan T., Sobha K., Veeraiah K. and Tilak K.S., Studies on biochemical changes in the tissues of Labeo rohita and Cirrhinus mrigala exposed to fenvalerate technical grade, J. Toxicol. Environ. Health Sci., 2(5), 53-62 (2010)
  28. Jenkins F., Smith V., Rajanna B., Shameem U., Umadevi K., Sandhya V. and Madhavi R., Effect of sub-lethal concentrations of endosulfan on haematological and serum biochemical parameters in the carp, Cyprinus carpio, Bull. Environ. Contam. Toxicol.,70(5),993-997 (2003)
  29. Singh N.N., Das V.K. and Singh S., Effect of aldrin on carbohydrate, protein and ionic metabolism of a fresh water catfish, Heteropneustes fossilis, Bull. Environ. Contam. Toxicol.,57, 204-210 (1996)