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Invitro Antibacterial activity of Arq e Ajeeb on Eschereschia Hirae, Eschereschia Coli, Pseudomonas Aeruginosa and Streptococcus Mutans

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Author Affiliations

  • 1Dept of Ilmul Amraz, (Pathology), Government Unani Medical College Bangalore, Karnataka, India
  • 2Dept. of Ilmul Saidala, (Pharmacy), Government Unani Medical College Bangalore, Karnataka, India
  • 3Dept of Ilmul Amraz, (Pathology), Government Unani Medical College Bangalore, Karnataka, India
  • 4Dept. of Kulliyat, Government Unani Medical College Bangalore, Karnataka, India

Int. Res. J. Medical Sci., Volume 12, Issue (1), Pages 1-4, February,28 (2024)

Abstract

The increase in the use of antibiotics and its resistance towards the micro organisms is a matter of great concern to the public health. Although many herbals drugs have been validated for antimicrobials activity, yet it is the need of the hour to figure out a few more antimicrobials. The Unani system of medicine have number of herbs and formulations which are used in treatment of many infections, one such formulation is Arq e Ajeeb(AA) which was used during epidemics for treatment in condition like Cholera (Haiza), Sil (Tuberculosis), Plague (Taoon), Seasonal fevers, and also it was used as anti inflammatory and analgesic. The ingredients of AA have shown antibacterial; hence AA was tested for its synergistic antimicrobial activity. A volume of 100μl sterile MHB broth for bacteria (P. aeruginosa, E. hirae, E. coli, S. mutans) was taken, Test sample of 100% concentration was used with double dilutions ranging from 100 – 0.048828mg. The experiment was repeated with Ciprofloxacin at 0.1% as Standard antibiotic. A growth control (Bacterial suspension + 100μl broth medium) and negative control (only broth medium 100μl) was taken. The plates were then incubated at 32.5±2.5°C for 24 to 48 hours. After incubation, 20μl of working solution of resazurin dye was added to all wells. The plates were wrapped with aluminum foil and incubated for 1hour. The color change was then assessed visually. Any colour change from purple to pink or colorless was recorded as positive (growth). MIC value was taken at lowest concentration at which there was no colour change. AA showed antibacterial activity at concentration 100 to 0.048828mg. MIC for Escherescia hirae, Eschereschia Coli., Streptococcus Mutansis at 0.048828mg. MIC for Pseudomonas aeruginosais at 1.562mg. AA can be used as a better alternative to antibacterial drugs, and it has shown antibacterial activity , hence further invitro antimicrobial on other microbes can be undertaken, and as AA ingredients have shown antifungal and antiviral properties, further studies may be undertaken.

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