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Assay of amoxicillin trihydrate and bromhexine hydrochloride by UV-spectrophotometric third order derivative method in combined dosage form

Author Affiliations

  • 1Central research laboratory, D.G. Ruparel College, Matunga, Mumbai 400016, India

Res. J. of Pharmaceutical Sci., Volume 6, Issue (4), Pages 1-5, June,30 (2017)

Abstract

A new economical and sensitive derivative, UV spectrophotometric, third order method is proposed for the validation of amoxicillin and bromhexine hydrochloride. Tablet dosages. The ICH guidelines were used for validation. The validation involves the study of optical density of two component at zero crossing point of each others. Therefore 220 nm and 236.2 nm wavelengths were found to be suitable for the assay of bromhexine hydrochloride and amoxicillin tri-hydrate respectively. A concentration range of 1 to 14 g /ml (r2= 0.9991) for bromhexine hydrochloride and 10 to 100 g /ml (r2= 0.9999) for amoxicillin showed the linearity study of the proposed method respectively. The 100.2 % for bromhexine hydrochloride and 100.15 % for amoxicillin were found as RSD values respectively. A statistical studies was used for validation for its accuracy, linearity as well as for precision. A less percentage (%) of RSD values for study of intra and inter day variations showed higher degree of precision.

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