International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

In-Vitro and In-Vivo Evaluation of Anti Asthmatic Activity of Roots Extract of Acorus Calamus (Linn.) in Guinea Pigs

Author Affiliations

  • 1Department of Chemistry, Dr. Harisingh Gour University Sagar-470001, MP, INDIA

Res. J. of Pharmaceutical Sci., Volume 3, Issue (5), Pages 1-6, July,30 (2014)


The aim of the paper is to evaluate the anti-asthmatic possessions of ethanolic extract of Rhizomes of Acorus calmus (Linn) by retaining in-vivo and in-vitro screening models in Guinea pigs. In vivo experiment was done by inducing bronchus asthma in guinea pigs by exposing them to 0.2 % histamine aerosol produced by an ultra-sound nebulizer in an aerosol chamber. sacrifice of animals In vitro were done by cervical dislocation and carotid bleeding. The trachea was dissected out and transferred to a dish containing kerb’s solution (composition (g/l): NaCl (6.5), KCl (0.33), CaCl2 (0.26), MgSO47H2O (0.28), NaHCO3 (2.5), KH2PO4 (0.19) and glucose 5.0)and cut diagonally between the section of the cartilage and continuously aerated and maintained at 37 ± 1°C. The trachea was adjourned and allowed to stabilize for at least 40 minutes. During stabilization, the bath was full with fresh kerb’s solution for every 15 minutes. As a results the ethanolic extract produced significant dose-dependent antiasthmatic activity. The extracts also inhibited the contraction induced by histamine, Primary phytochemical screening revealed the presence of flavonoids, steroids, saponins, and tannins. The present study confirmed that the Rhizomes extract of Acorus Calamus exhibited bronchodilator activity.


  1. Achliya G.S., Wadodkar S.G., and Dorle A. K. Evaluation of CNS activity of Bramhi Ghrita, Indian J. Pharmacol., 37, 33-36 (2005)
  2. Aqil F., and Ahmad I., Antibacterial properties of traditionally used Indian medicinal plants, Methods Find, Exp. Clin. Pharmacol., 29, 79-92 (2007)
  3. Elaya R. A., Vijayalakshmi M., and Devalara G., Acorus calamus linn.: Chemistry and Biology, Res. J. Pharm. and Tech.,2 (2), 256-261 (2009)
  4. Harish M.S., Nagur M., and Badami S., Antihistaminic and mast cell stabilizing activity of Striga orobanchioide, J Ethnopharmacol, 76, 197-200 (2001)
  5. Bigoniya1 P., Shukla A., Agrawal G.P. and Rana A.C., Pharmacological Screening of Wrightia tinctoria Bark Hydro-Alcoholic Extract, Asian J. Exp. Sci.,22( 3), 235-244 (2008)
  6. Afreen A., Kashyap P., Sawarkar H., Deshmukh V., Upadhyay A. and Pal S., In- vitro and In- vivo Models for Evaluation of Anti-Asthmatic Activity: A Review,International Journal of Herbal Drug Research, I(1), 19-27(2011)
  7. Surendra A., Pusapati M.R. and Harish M.S., Antiasthmatic Activity Of Aqueous Extract Of Pistacia Integerrima Galls, International Journal of Pharmacy and Pharmaceutical Sciences, 5(2), 116-121(2013)
  8. Kumar D., Bhujba S. S., Deoda R. S., and Mudgade S. C., In-vitro and In-vivo Antiasthmatic Studies Ailanthus excelsa Roxb. on of Guinea Pigs, Journal of scientific research2(1), 196-202 (2010)
  9. Kulkarni S.K. Hand book of experimental pharmacology, Vallabh Prakashan, New Delhi, II Edn, 22. (1979)
  10. Tejas P. and Samir S., Anti asthmatic activity of aqueous extract of Myrica nagi bark, Journal of Current Pharmaceutical Research, 10 (1), 34-39 (2012)
  11. Kajaria D., Kajaria A., Tripathi J. S., and Tiwari S.K, In-Vitro and In-Vivo Assessment of Anti-Asthmatic Activity of Polyherbal Ayurvedic Drug, Journal of Pharmacy and Biological Sciences, 6( 3), 60-70 (2013)
  12. Stephen O Okpo, Gerald I Eze, Ifeanyi H Ajaanonwu1,Ogochukwu L Ijei,Dickson O Uwaya, Viona Ologe, Evaluation of the Anti-asthma Activity of Aqueous Root,Bark Extract of Ficus exasperata Vahl (Moraceae), International Journal of Health Research, 5(1), 5-12 (2012)
  13. Sharma P., Hullatti K.K., Kuppasth I.J. and Sharma S., Studies on Anti-Asthmatic Property Of Cyamopsis Tetragonoloba (L.) Taub. Leaf Extracts, Journal of Natural Remedies, 10(1), 81–86 (2010)
  14. Garg M and Garg C, Effect of Phyllanthus Urinaria in Biochemical Profile of Experimental Hyperglycemic Albino Rats, es. J. Pharmaceutical Sci,1(1), 2-5(2012)
  15. Sharma A., Bhatia S., Kharya M.D., Gajbhiye V., Ganesh N.A.G., Namdeo K.R. and Mahadik, Anti-inflammatory and analgesic activity of different fractions of Boswellia serrate, International Journal of Phytomedicine,2, 94-99 (2010)
  16. Nalamwar V.P., Khadabadi S.S., Aswar P.B., Kosalge S.B. and Rajurkar R.M. In vitro Licicidal Activity of Different Extracts Of Acorus calamus Linn. (Araceae) Rhizome.International Journal of PharmTech Research 1(1), 96-100 (2009)
  17. Shelke M.E., S-Triazines: As Alternative Drugs for the Treatments of Typhoid, Res. J. of Pharmaceutical Sci.,2(1), 18-19(2013)
  18. Parganiha R., Verma S, Chandrakar S., Pal S., Sawarkar H.A. and Kashyap P., In vitro anti- asthmatic activity of fruit extract of Piper nigrum (Piperaceae), International Journal of Herbal Drug Research, 1(I), 15-18(2011)
  19. Anbu Jeba Sunilson J.,Anandarajagopa , K., Abdullah khan, Khaja Pasha, Qusro Bin Hassan and Puspa V. Kuna Raja. Antihistaminic evaluation of formulated polyherbal cough syrup, Journal of Medicinal Plants Research,4(14), 1482-1485, (2010)
  20. Neelmani C., Saurabh R. and Dubey B.K., Antiasthmatic Effect of Rhizomes of Clitorea Ternatea LinnInternational Journal of Pharmaceutical and research,3(4), 1076-1079, (2012)