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In-Vitro and In-Vivo Evaluation of Anti Asthmatic Activity of Roots Extract of Acorus Calamus (Linn.) in Guinea Pigs

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Author Affiliations

  • 1Department of Chemistry, Dr. Harisingh Gour University Sagar-470001, MP, INDIA

Res. J. of Pharmaceutical Sci., Volume 3, Issue (5), Pages 1-6, July,30 (2014)

Abstract

The aim of the paper is to evaluate the anti-asthmatic possessions of ethanolic extract of Rhizomes of Acorus calmus (Linn) by retaining in-vivo and in-vitro screening models in Guinea pigs. In vivo experiment was done by inducing bronchus asthma in guinea pigs by exposing them to 0.2 % histamine aerosol produced by an ultra-sound nebulizer in an aerosol chamber. sacrifice of animals In vitro were done by cervical dislocation and carotid bleeding. The trachea was dissected out and transferred to a dish containing kerb’s solution (composition (g/l): NaCl (6.5), KCl (0.33), CaCl2 (0.26), MgSO47H2O (0.28), NaHCO3 (2.5), KH2PO4 (0.19) and glucose 5.0)and cut diagonally between the section of the cartilage and continuously aerated and maintained at 37 ± 1°C. The trachea was adjourned and allowed to stabilize for at least 40 minutes. During stabilization, the bath was full with fresh kerb’s solution for every 15 minutes. As a results the ethanolic extract produced significant dose-dependent antiasthmatic activity. The extracts also inhibited the contraction induced by histamine, Primary phytochemical screening revealed the presence of flavonoids, steroids, saponins, and tannins. The present study confirmed that the Rhizomes extract of Acorus Calamus exhibited bronchodilator activity.

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