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Effect of Aluminum on different parts of Brainstem of Old Rats: Haematological, Biochemical and Morphological Study

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Author Affiliations

  • 1Department of Advance Science, Nims Institute of Engineering and Technology, NIMS University, Jaipur INDIA
  • 2 Department of Biochemistry, King George’s Medical University, Lucknow, INDIA

Res. J. of Pharmaceutical Sci., Volume 2, Issue (3), Pages 6-11, May,30 (2013)

Abstract

Aluminum (Al) has identified one of the environmental factors that may cause several neurodegenerative disorders. Generally, uptake of aluminum is greater the older a person becomes. We have orally administered 100 mg AlCl3 (by gavage) to +24 m male rats (n=10) for 90 days. The same number of age and sex matched rats were concurrently administered equal volume of physiological saline. Hematological and serum lipid profile were investigated on 45 and 90 days of Al exposure. Medulla, pons and mid brain were selected for biochemical investigations ie., lipid peroxide levels (LPx), superoxide dismutase (SOD), catalase and glutathione (GSH). Results showed that AlCl3 increases LPO level while the activity of antioxidant enzymes and glutathione was decreased significantly in all the part of brain stem along with decreased activity of antioxidant enzyme. On the basis of results it may conclude that hematology and lipid profiles may be the markers of Al neurotoxicity and mid brain and medulla is more affected area of the brainstem.

References

  1. Hounkpatin A.S.Y., Johnson R.C., Guédénon P., Domingo E., Alimba C.G., Boko M. and Edorh P.A., Protective Effects of Vitamin C on Haematological Parameters in Intoxicated Wistar Rats with Cadmium, Mercury and Combined Cadmium and Mercury, Int. Res. J. Biological Sci.,1(8), 76-81 (2012)
  2. Akinyeye A.J. and Okorie T.G., Heavy Metal Studies of Industrial Effluent on Alaro Stream Sediment, Int. Res. J. Biological Sci.,1(6), 1-9 (2012)
  3. ATSDR (Agency for Toxic Substances and Disease Registry), Toxicological Profile for Aluminum (Update), U.S. Department of Health and Human Services, Public Health Service(1999)
  4. Mishra P.C., Dash A.K. and Khageswar P., Metals in Environmental segments at Hirakud of Odisha, India Int Biological Sci.,1(1), 7-23 (2012)
  5. Yokel, R.A., Blood-brain barrier flux of aluminum, manganese, iron and other metals suspected to contribute to metal-induced neurodegeneration, J Alzheimers Dis., 10(2-3), 223-53 (2006)
  6. Saranya A.V., and Ravi S., In-vitro Acetylcholine Esterase Inhibition activity of Chalcones with Phenothiazine Moiety, Res. J. Recent Sci.,1(12), 40-43 (2012)
  7. Tripathi S., Mahdi A.A., Nawab A., Chander R., Hasan M., Mahdi F., Mitra K. and Bajpai V.K., Influence of age on aluminium induced lipid peroxidation and neurolipofuscin in frontal cortex of rat brain: A behavioral, biochemical and ultrastructural study, Brain Res.,1253, 107-116 (2009)
  8. Sakamoto M., Wakabayashi K., Kakita A., Takahashi H., Adachi T. and Nakano A., Widespread neuronal degeneration in rats following oral administration of methylmercury during the postnatal developing phase: a model of fetal-type Minamata disease, Brain Research,784(1-2), 351-354 (1998)
  9. Haribhau M.G., Trace Metals Contamination of Surface Water Samples in and Around Akot City in Maharashtra, India, Res.J.Recent Sci., 1(7), 5-9 (2012)
  10. Lowry O.H., rosebrough N.J., Farr A.L. and Randall R.J., protein measurement with the Folin phenol reagent, J.Biol. Chem., 193, 265-268 (1951)
  11. Ohkawa H., Ohishi N. and Yagi K., Assay for lipid peroxides in animal tissue by thio-barbituric acid reaction, Anal. Biochem., 95(2), 351-358 (1979)
  12. McCord J.M. and Fridovich I., Superoxide dismutase: An enzyme function for erythrocuprin, J Biol Chem., 244(22), 6049-55 (1969)
  13. Aebi H., Catalase, In: Methods of Enzymatic Analysis. H.U. Bergmeyer, editor, Academic Press Inc New York. 2(1), 673-684 (1974)
  14. Ellman G.L., Tissue sulfydryl groups, Arch Biochem Biophysics,82(1), 70 (1959)
  15. Ellman G.L.V., Courtney R.M. and Andres, A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem Pharmacol, 7(1), 88–95 (1961)
  16. Tripathi S., Mahdi A.A., Hasan M., Mahdi F. and Bajpai V.K., Protective potential of Bacopa monniera extract on aluminum induced cerebellum toxicity and neuromuscular decline in aging rats, Cell Mol Bio., 57(1), 3-15 (2011)
  17. Pal A., Choudhary M., Joshi D.K., Tripathi S. and Modi D.R., Alteration in Thyroid Hormones and Vitamins As Early Markers of Aluminum Induced neurodegeneration in Rats, Int. J. Res. Pharm. Sci., 2(2), 137-150 (2012)
  18. Mahieu S., del Carmen Contini M., Gonzalez M., Millen N. and Elias M.M., Aluminum toxicity. Hematological effects, Toxicol Lett.,111(3), 235-42 (2000)
  19. Deloncle R., Huguet, F., Fernandez, B., Quellard, N., Babin, P.H. and Guillard, O., Ultrastructural study of rat hippocampus after chronic administration of aluminum l-glutamate: an acceleration of the aging process, Experimental Gerontology, 36(2), 231–244 (2001)
  20. Farina M., Lara F.S., Brandao R., Jacques R. and Rocha J.B.T., Effects of aluminum sulfate on erythropoiesis in rats, Toxicol Lett., 132(2), 131-139 (2002)
  21. Shafiq-Ur-Rehman, Lead-induced regional lipid peroxidation in brain, Toxicology Lett., 21(3) 333–337 (1984)
  22. Sushma J.N., Sivaiah U., Suraj N.J. and Rao K.J., Aluminium Acetate Induced Oxidative Stress in Brain of Albino Mice, Journal of Pharm. Toxicol., 1(6) 579-584 (2006)
  23. Liu R. and Liu I.Y., Thompson R.F., Doctrow S.R., Malfroy B. and Baudry M., Reversal of age related learning and brain oxidative stress in mice with superoxide dismutase/catalase mimetics, Proc Natl Acad Sci USA. 100(14), 8526–8531 (2003)
  24. Roskams A.J. and Connor J.R., Aluminium access to the brain: a role for transferrin and its receptors. Proc Natl Acad Sci USA, 87(22), 9024–9027 (1990)
  25. Campbell A., Becaria A., Lahiri D.K., Sharman K. and Bondy S.C., Chronic exposure to aluminium in drinking water increases inflammatory parameters selectively in the brain, J Neurosci Res., 75(4), 565–572 (2004)
  26. Andresen M.C. and Kunze D.L., Nucleus tractus solitarius– gateway to neural circulatory control, Annu Rev Physiol,56, 93–116 (1994)
  27. Sakai K., Hirooka Y., Shigematsu H., Kishi T., Ito K., Shimokawa H., Takeshita A. and Sunagawa K. Overexpression of eNOS in brain stem reduces enhanced sympathetic drive in mice with myocardial infarction, Am J Physiol Heart Circ Physiol.,289(5), H2159-2166 (2005)
  28. Hirooka Y, Shigematsu H, Kishi T, Kimura Y, Ueta Y. and Takeshita A., Reduced nitric oxide synthase in the brainstem contributes to enhanced sympathetic drive in rats with heart failure, J Cardiovasc Pharmacol., 42 (1), S111-115 (2003)
  29. Hegde L.G., Shukla R., Srimal R.C. and Dikshit M. , Attenuation in rat brain nitric oxide synthase activity in the coarctation model of hypertension, Pharmacol Res.,36(2), 109-14 (1997)