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Validation of Modified QuEChERS method using Low Cost Adsorbent and Screening of Selected Organochlorine and Organophosphorus Insecticides in Fruits and Vegetables

Author Affiliations

  • 1Food Safety and Analytical Quality Control Laboratory, CSIR-Central Food Technological Research Institute, Mysore - 570020, INDIA
  • 2 G.D. Rungta College of Science and Technology affiliated to Pt. Ravishankar University, Raipur 490023, INDIA

Int. Res. J. Environment Sci., Volume 4, Issue (5), Pages 18-27, May,22 (2015)


A highly economical and simple sample preparation technique for the analysis of insecticide residues in fruits and vegetables was developed by modifying the existing Quick Easy Cheap Efficient Rugged and Safe (QuEChERS) technique and a full in-house validation was carried out for different pesticides belonging to organochloride and organophosphorus groups. In this newly developed sample preparation technique, easily available low cost fly ash which is a sugarcane industry waste was used as an adsorbent in place of expensive Primary Secondary Amine (PSA). Efficiency of the modified method was compared with that of original QuEChERS method by conducting parallel experiments. Insecticides were investigated at levels 0.01΅g/g in fruits (apple, grape and orange) and vegetables (cabbage, cauliflower, tomato and brinjal) using low cost adsorbent. Validation study fulfilled the requirement of SANCO guideline 2012. Experiments proved that the modified method can be adopted for routine monitoring of insecticide residues (dimethoate, malathion, alfa and beta-endosulfan) in fruits and vegetables. Out of seventy samples of fruits and vegetables collected from different outlets, Eleven samples were found to be contaminated with insecticides. Though residues were determined in tomato, cabbage, grape, orange and apple, they did not exceed the Maximum Residue Limit (MRL) prescribed under Food Safety Standard Authority of India. However, out of ten samples of cabbage analyzed, only one sample found to contain dimethoate residue, three times more the permitted level specified under Codex.


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