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Bacillus Thuringiensis as Endophyte of Medicinal Plants: Auxin Producing Biopesticide

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Author Affiliations

  • 1Dept. of Microbiology, Ramnarain Ruia College, Matunga, Mumbai 400019, INDIA

Int. Res. J. Environment Sci., Volume 3, Issue (9), Pages 27-31, September,22 (2014)

Abstract

Occurrence of Bacillus thuringiensis, a biopesticide living as endophytes in the leaves of medicinal plant and their ability to produce Indole-3-acetic acid was studied. Phenotypic characterization identified the endophytes as Bacillus sp. The presence of parasporal crystalline inclusion was observed using phase contrast microscopy for all the isolated endophytic Bacilli. Coomassie brilliant blue (CBB) staining further established the presence of endospore and parasporal body. Appearance of insecticidal proteins (ICP) in the sporulation phase observed as a dark stained blue structure confirmed the endophytes as B. thuringiensis. Genotypic variation was studied by 16S rRNA gene amplification and sequencing. All isolated endophytic B. thuringiensis were observed to have the ability to produce phytohormone Indole-3-acetic acid. This study shows the presence and association of endophytic biopesticide with its host medicinal plant and its potential to produce an important plant growth hormone. This unique finding of such naturally occurring association is of great value to research as well as agricultural industry.

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