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Isolation of Broad Spectrum Antibiotic-Resistant Bacteria from Waste Water using 16s rDNA Technology

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Author Affiliations

  • 1Department of Environmental Science & Engineering, Guru Jambheshwar University of Science & Technology, Hisar, Haryana, India
  • 2UK Center for Ecology & Hydrology, Benson Lane, Wallingford, United Kingdom
  • 3Department of Environmental Science & Engineering, Guru Jambheshwar University of Science & Technology, Hisar, Haryana, India
  • 4Maharshi Dayanand University, Rohtak, Haryana, India

Int. Res. J. Environment Sci., Volume 11, Issue (4), Pages 13-17, October,22 (2022)

Abstract

This study focuses on multiple drug resistance (MDR) bacteria prevalence in wastewater treatment plants specifically in domestic and medical college sewage treatment plants (STP) in Hisar district of Haryana, India. MDR was identified from wastewater samples using broth dilution and the Kirby Bauer disk diffusion method. Results obtained from these methods showed 100% resistance against Ampicillin (AMP), Vancomycin (VAN), Tetracycline (TET) and Rifampicin (RIF). Variable susceptibility was checked for other antibiotics like 60% to 51.9% for Chloramphenicol (CHL), 53% to 43.2% for Cephotaxime (CTX), 47% to 39% for Streptomycin (STR), 21% to 15.7% for Trimethoprim (TMP) and 27.1% to 13% for Ceftazidime (CAZ). Isolated antibiotic-resistant bacteria were identified by using 16s rDNA technology.

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