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Isolation of bioactive compounds by GC-MS and biological potentials of acanthus ilicifolius, L.

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Author Affiliations

  • 1Dept. of Botany, St. Teresa’s College, Ernakulam-682011, Kerala, India
  • 2Dept. of Botany, St. Teresa’s College, Ernakulam-682011, Kerala, India

Int. Res. J. Biological Sci., Volume 6, Issue (6), Pages 7-19, June,10 (2017)

Abstract

Mangroves are ecological group of halophytic plant species, which provide a wide range of pharmaceutical and economic products and services. The present work focussed on a study of Acanthus ilicifolius, L.a mangrove plant from Kerala. Phytochemical constituents were determined by UV-Visible spectrophotometer. Free radical scavenging activity was estimated using in vitro methods like DPPH, Nitric Oxide Radical, ABTS and Super Oxide Dismutase while minimum inhibitory concentration of leaf extracts were determined by resazurin based microtiter dilution assay and the characterisation of bioactive components by GC-MS analysis. The methanol extract of A. ilicifolius, L. leaf showed better scavenging activity in SOD method (419 µg/ml) followed by ABTS (450.7 µg/ml), NO (556 µg/ml) and DPPH (562.5 µg/ml). The crude methanolic extract showed remarkable MIC of 0.469 mg/ml against Staphylococcus aureus, 0.521 mg/ml against Klebsiella pneumonia, 0.416 mg/ml against Candida albicans and Pencilliumnotatum. Phenols, flavonoids and tannins had higher correlation with antioxidant activities. The GC-MS of active column fraction revealed that the active principles were a mixture of Lupeol, decanoic acids, cyclolignan glycosides, glycine, cyano colchicine and other therapeutically active compounds. It could be concluded that A .ilicifolius, L. contains compounds with various biological activities and so recommended as a plant of phyto pharmaceutical importance.

References

  1. Saranya Arumugam, Ramanathan T., Kesavanarayanan K.S. and Adam A. (2015)., Traditional Medicinal Uses, Chemical Constituents and Biological Activities of a Mangrove Plant, Acanthus ilicifolius, Linn. : A Brief Review., J. Agric. & Environ. Sci., 15(2), 243-250.
  2. Ganesh S. and Vennila J.J. (2010)., Screening for antimicrobial activity in Acanthus ilicifolius., Arch. Appl. Sci. Res, 2(5), 311-315.
  3. Wostmann R. and Liebezeit G. (2008)., Chemical composition of the mangrove holly Acanthus ilicifolius (Acanthaceae) - review and additional data., Senckenbergiana Maritime, 38, 31-37.
  4. Liu L., Fan H., Qi P., Mei Y., Zhou L., Cai L., Lin X. and Lin J. (2013)., Synthesis and hepatoprotective properties of Acanthus ilicifolius alkaloid A and its derivatives., Exp. Ther. Med, 6(3), 796-802.
  5. Govindasamy C. and Kannan R. (2012)., Pharmacognosy of mangrove plants in the system of unani medicine., Asian Pac J Trop Dis, 2(Suppl 1), S38-S41.
  6. Firdaus M., Prihanto A.A. and Nurdiani R. (2013)., Antioxidant and cytotoxic activity of Acanthus ilicifolius flower., Asian Pac J Trop Biomed, 3, 17-21.
  7. Mani Senthil Kumar K.T., Gorain B., Roy D.K., Samanta S.K., Pal M., Biswas P., Roy A., Adhikari D., Karmakar S. and Sen T. (2008)., Anti-inflammatory activity of Acanthus ilicifolius., J Ethnopharmacol., 120, 7-12.
  8. Babu B.H., Shylesh B.S. and Padikkala J. (2001)., Antioxidant and hepatoprotective effect of Acanthus ilicifolius., Fitoterapia., 72(3), 272-277.
  9. Wu J., Zhang S.I., Li Q., Huang J., Xiao Z. and Long L. (2004)., Two New Cyclolignan Glycosides from Acanthus ilicifolius., Zeitschrift für Naturforschung B, 59(3), 341-344.
  10. Kanchanapoom T., Kamel M.S., Kasai R., Yamasaki K., Picheansoonthon C. and Hiraga Y. (2001)., Lignanglucosides from Acanthus ilicifolius., Phytochemistry, 56(4), 369-372.
  11. Huo C., Liang H., Tu G., Zhao Y. and Lin W.A. (2008)., New 5, 11 epoxy megastigmaneglucoside from Acanthus ilicifolius., Nat. Prod. Res, 22(10), 896-900.
  12. Gamble J.S. (1984)., Flora of the presidency of Madras., Adlard & Son Ltd, 21, Hart Street, W.C. London, 1014.
  13. Harborne J.B. (1973)., Phytochemical Methods., Chapman and Hall Ltd, London. 49-188.
  14. Sofowora A. (1982)., Medicinal plants and traditional medicine in Africa., New York John Wiley 1982, 256.
  15. Trease G.E. and Evans W.C. (1989)., A test book of pharmacognosy, 11th (Ed.) Bailliere Tindall., London, 430.
  16. Omoruyi B.E., Bradley G. and Afolayan A.J. (2012)., Antioxidant and phytochemical properties of Carpobrotusedulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province., Complementary and Alternative Medicine, 12, 215.
  17. Boham A.B. and Kocipai R.A. (1974)., Flavonoid and condensed tannins from leaves of Hawaiian vaccinumvaticulum and vicalycimum., PracificSci, 48, 458-463.
  18. Chang C., Yang M., Wen H. and Chern J. (2002)., Estimation of total Flavonoids content in Propilis by two complementary colorimetric methods., J. Food Drug Analysis, 10(3), 178-182.
  19. Lu Y. and Foo L.Y. (2001)., Antioxidant activities of polyphenols from sage (Salvia officinalis)., Food Chemistry, 75(2), 197-202.
  20. Kono Y. (1978)., Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase., Arch. Biochem. Biophys., 186(1),189-195.
  21. Garratt D.C. (1964)., The quantitative analysis of drugs., Third Edition, Chapman and Hall Ltd., Tokyo, 456-458.
  22. Re R., Pellegrini N., Proteggente A., Pannala A. and Yang M. (1999)., Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolourization assay., Free RadicBiol Med, 26(9), 1231-1237.
  23. Palomino J.C., Martin A., Camacho M., Guerra H., Swings J. and Portaels F. (2002)., Resazurinmicrotiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis., Antimicrob Agents Chemother, 46(8), 2720-2722.
  24. Wadood A., Ghufran M., Jamal S.B., Naeem M., Khan A., Ghaffar R. and Asnad C. (2013)., Phytochemical Analysis of Medicinal Plants Occurring in Local Area of Mardan., Biochem Anal Biochem, 2(4), 1-4.
  25. Satapathy S., Satapathy S. and Jena B.K. (2013)., Antitumor and Growth effector screen of leaf extracts of selected mangroves of Bhitarkanika, Odisha., International Journal of Technology Enhancements and Emerging Engineering Research,1(4), 25-30.
  26. Krishnamoorthy M., Sasikumar J.M., Shamna R., Pandiarajan C., Sofia P. and Nagarajan B. (2011)., Antioxidant activities of bark extract from mangroves, Bruguiera cylindrical (L.) Blume and Ceriops decandra Perr., Indian J Pharmacol., 43(5), 557-562.
  27. Cartea M.E., Francisco M., Soengas P. and Velasco P. (2011)., Phenolic compounds in Brassica vegetables., Molecules, 16, 251-280.
  28. Shelar P.S., Reddy V.K., Shelar G.S. and Reddy G.V.S. (2012)., Medicinal value of mangroves and its antimicrobial properties – A REVIEW., Continental J. Fisheries and Aquatic Science, 6(1), 26-37.
  29. Ayala-Zavala J.F., Silva-Espinoza B.A., Cruz-Valenzuela M.R., Villegas-Ochoa M.A., Esqueda M., González-Aguilar G.A. and Calderón-López Y. (2012)., Antioxidant and antifungal potential of methanol extracts of Phellinusspp. from Sonora, Mexico., RevistaIberoamericana De Micología. 29(3), 132-138.
  30. Vaya J., Belinky P.A. and Aviram M. (1997)., Antioxidant constituents from licorice roots; Isolation, structure elucidation and antioxidative capacity toward LDL oxidation., Free Rad. Biol. Med, 23(2), 302-313.
  31. Robbins R.J. (2003)., Phenolic acids in foods: an overview of analytical methodology., J Agric Food Chem, 51(10), 2866-2887.
  32. Chandra P. and Arora D.S. (2012)., Optimization of antioxidant potential of Penicillium granulatum Bainier by statistical approaches., Microbiol., 1-10.
  33. Gardner P.T., White T.A.C., McPhail D.B. and Duthie G.G. (2000)., The relative contributions of vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices., Food Chem., 68(4), 471-474.
  34. Hamid A.A., Aiyelaagbe O.O., Usman L.A., Ameen O.M. and Lawal A. (2010)., Antioxidants: Its medicinal and pharmacological applications., Afri. J. Pure Appl. Chem. 4(8), 142-151.
  35. Sram R.J., Binkova B. and Rossner P. (2012)., Vitamin C for DNA damage prevention., Mutat. Res.Fundamental Mol. Mech. Mutagenesis. 733, 39-49.
  36. McNicholl B.P., McGrath J.W. and Quinn J.P. (2006)., Development and application of a resazurin-based biomass activity test for activated sludge plant management., Water Res, 41,127-133.
  37. Bigalke D.L. (1984)., Methods used for monitoring the microbiological quality of raw milk., Dairy Food Sanit, 4, 189-190.
  38. Ganesh S. and Jannet Vennila J. (2010). Scholars Research Library, 2(5), 311-315., undefined, undefined
  39. Manilal A., Sujith S., Kiran G.S., Selvin J. and Shakir C. (2009)., Biopotentials of mangroves collected from the Southwest coast of India., Global J. Biotechnol. Biochem., 4, 59-65.
  40. Bose S. and Bose A. (2008)., Antimicrobial Activity of Acanthus ilicifolius (L.)., Indian J Pharm Sci., 70(6), 821-823.
  41. Graham J.G., Quinn M.L., Fabricant D.S. and Farnsworth N.R. (2000)., Plants used against cancer., J Ethnopharmacol,73(3), 347-377.
  42. Babu B.H., Shylesh B.S. and Padikkala J. (2002)., Tumour reducing and anticarcinogenic activity of Acanthus ilicifoliusin mice., J Ethnopharmacol,79, 27-33.
  43. Tiwari P., Kumar B., Kaur M., Kaur G. and Kaur H. (2011)., Phytochemical screening and extraction., Int Pharm Sci, 1, 98-106.
  44. Firdaus M., Prihanto A.A., Nurdiani R. and Widodo N. (2013)., Antioxidant and cytotoxic activity of Acanthus ilicifolius flower., Asian Pac J Trop Biomed, 3, 17-21.
  45. Sen S., De B., Devanna N. and Chakraborty R. (2013)., Total phenolic, total flavonoid content, and antioxidant capacity of the leaves of Meynaspinosa Roxb., an Indian medicinal plant., Chin J Nat Med, 11, 149-157.
  46. Saeed N., Khan M.R. and Shabbir M. (2012)., Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophyllaL., BMC Complement Altern Med, 12, 212.
  47. Saikia L.R. and Upadhyaya Sristisri (2011)., Antioxidant activity, phenol and flavonoid content of some less known medicinal plants of Assam., International Journal of Pharma and Bio Sciences, 2(2), 383-388.
  48. Jimoh F.O., Adedapo A.A., Aliero A.A. and Afolayan A.J. (2008)., Polyphenolic Contents and Biological Activities of Rumex Ecklonianus., Pharmaceutical Biology, 46(5), 333-340.
  49. Kumar Shashank and Pandey Abhay K. (2013)., Chemistry and Biological Activities of Flavonoids: An Overview., The Scientific World Journal, 1-16.
  50. Nigam S. and Schewe T. (2000)., Phospholipase A2s and lipid peroxidation., BiochemBiophyActa, 1488, 167-181.
  51. K. Satoh and H.Sakagami (1997)., Effect of metal ions on radical intensity and cytotoxic activity of ascorbate., Anticancer Research, 17(2A), 1125‐1129.
  52. Senevirathne Mahinda, Kim Soo-Hyun, Siriwardhana Nalin, Ha Jin-Hwan, Lee Ki-Wan and Jeon You-Jin (2006)., Antioxidant potential of Eckloniacavaon reactive oxygen species scavenging, metal chelating, reducing power and lipid peroxidation inhibition., Food Sci. Technol Int., 12, 27‐38.
  53. R. Govindarajan, kumar M. Vijaya, Rawat A.K.S. and Shanta M. (2003)., Free radical scavenging potential of Picorrhizakurroa Royle ExBenth., Ind J ExpBiol, 41, 875-879.
  54. Valko M., Leibfritz D., Moncol J., Cronin M.T.D., Mazur M. and Telser J. (2007)., Free radicals and antioxidants in normal physiological functions and human disease., Int J Biochem Cell Biol, 39, 44-84.
  55. Moncada S., Palmer R.M. and Hiiggs E.A. (1991)., Nitric oxide: Physiology, pathophysiology and pharmacology., Pharmacol Rev, 43(2), 109-142.
  56. Wu X., Beecher G.R., Holden J.M., Haytowitz D.B., Gebhardt S.E. and Prior R.L. (2004)., Lipophilic and hydrophilic antioxidant capacities of Common Foods in the United States., Journal of Agriculture and Food Chemistry, 52(12), 4026-4037.
  57. Valgimigli L., Banks J.T., Lusztyk J. and Ingold K.U. (1995)., Kinetic solvent effects on hydroxyl hydrogen atom abstractions are independent of the nature of the abstracting radical. Two extreme test using vitamin E and Phenol., Journal of the American Chemical Society, 117(40), 9966-9971.
  58. Prior R.L., Wu X. and Schaich K. (2005)., A standardized method for the determination of antioxidant capacity and phenolic in foods and dietary supplements., Journal of Agricultural and Food Chemistry, 53(10), 4290-4302.
  59. Fiedor J. and Burda K. (2014)., Role of Carotenoids as Antioxidants in Human Health and Disease., Nutrients, 6(2), 466-488.
  60. Bandaranayake W.M. (1998)., Traditional and medicinal uses of mangroves., Mangroves Salt Marshes, 2(3), 133-148.
  61. Govindasamy C. and Kannan R. (2012)., Pharmacognosy of mangrove plants in the system of unani medicine., Asian Pac J Trop Dis, 2(Suppl 1), S38-S41.
  62. Sundaram R., Ganesan R. and Murugesan G. (2012)., In vitro antiplasmodial activity of spirobenzofuran compound from mangrove plant of Southern India., Asian Pac J Trop Med,5(5), 358-361.
  63. Wöstmann R. and Liebezeit G. (2008)., Chemical composition of the mangrove holly Acanthus ilicifolius (Acanthaceae) - review and additional data., Senckenbergiana Maritime, 38, 31-37.
  64. Tiwara K.P., Minocha P.K. and Masood M. (1980)., Acanthicifoline - a new alkaloid from Acanthus ilicifolius., Chemischer Informationsdienst, 11, 48.
  65. Huo C.H., Wang B., Lin W.H. and Zhao Y.Y. (2005)., Benzoxazinones from Acanthus ilicifolius., BiochemSystEcol, 33(6), 643-645.
  66. Chinnavenkataraman Govindasamy and Mani Arulpriya (2013)., Antimicrobial activity of Acanthus ilicifolius: Skin infection pathogens., Asian Pac J Trop Dis, 3(3), 180-183.
  67. Wu Jun, Zhang S.I., Li Q., Huang J., Xiao Z. and Long L. (2004)., Two New Cyclolignan Glycosides from Acanthus ilicifolius., Zeitschrift für Naturforschung B, 59(3), 341-344.
  68. Wöstmann R. and Liebezeit G. (2008)., Chemical composition of the mangrove holly Acanthus ilicifolius (Acanthaceae) - review and additional data., Senckenbergiana Maritime, 38, 31-37.
  69. Babu B.H., Shylesh B.S. and Padikkala J. (2001)., Antioxidant and hepatoprotective effect of Acanthus ilicifolius., Fitoterapia., 72(3), 272-277.
  70. Firdaus M., Prihanto A.A. and Nurdiani R. (2013)., Antioxidant and cytotoxic activity of Acanthus ilicifolius flower., Asian Pac J Trop Biomed, 3, 17-21.
  71. Huo C., Liang H., Tu G., Zhao Y. and Lin W.A. (2008)., New 5, 11 epoxy megastigmaneglucoside from Acanthus ilicifolius., Nat. Prod. Res, 22(10), 896-900.
  72. Kanchanapoom T., Kasai R. and Yamasaki K. (2002)., Flavonoid Glycosides from Acanthus ilicifoliusL., Nat. Med, 56(3), 122.
  73. Park E.S., Moon W.S., Song M.J., Kim M.N., Chung K.H., and Yoon J.S. (2001)., Antimicrobial activityof phenol and benzoic acid derivatives., IntBiodeterior Biodegradation, 47(4), 209-14.
  74. Mishra P.M. and Sree A. (2007)., Antibacterial activity and GCMS analysis of the extract of leaves of Finlaysoniaobovata (a mangrove plant)., Asian J Plant Sci, 6(1), 168-172.
  75. Chandrasekar T., Rao M.R., Kumar R.V., Prabhu K., Kumar S.N. and Divya D. (2015)., GC-MS analysis, antimicrobial, antioxidant activity of an Ayurvedic medicine, Nimbapatradi Choornam., J Chem Pharm Res, 7(8), 124-36.
  76. Togashi N., Shiraishi A., Nishizaka M., Matsuoka K., Endo K. and Hamashima H. (2007)., Antibacterial activity of long-chain fatty alcohols against Staphylococcus aureus., Molecules, 12(2), 139-48.
  77. Tajkarimi M. and Ibrahim S.A. (2011)., Antimicrobial activity of ascorbic acid alone or in combination with lactic acid on Escherichia coli O157: H7 in laboratory medium and carrot juice., Food Control, 22(6), 801-804.
  78. Uma B. and Parvathavarthini R. (2010)., Antibacterial effect of hexane extract of sea urchin, Temnopleurusalexandri (Bell,1884)., Int J PharmTech Res, 2(3), 1677-1680.
  79. Canas-Rodriguez A. and Smith H.W. (1966)., The identification of the antimicrobial factors of the stomach contents of sucking rabbits., Biochem J, 100(1), 79-82.
  80. Al-Bari M.A., Sayeed M.A., Rahman M.S. and Mossadik M.A. (2006)., Characterization and antimicrobial activities of a phthalic acid derivative produced by Streptomyces bangladeshiensis - A novel species collected in Bangladesh., Res J Med Med Sci, 1(2), 77-81.
  81. Ajoke F.L., Kaita H. and Ilyas M. (2014)., Antibacterial Activity of 1,2-benzenediccarboxylic acid, dioctyl ester isolated from the ethyl acetate soluble sub-portion of the unripe fruits of Nauclealatifolia., Int J Pure App Biosci, 2(1), 223-230.
  82. Ko T.F., Weng Y.M. and Chiou R. (2002)., Squalene content and antioxidant activity of Terminaliacatappaleaves and seeds., J Agric Food Chem, 50(1), 5343-5348.
  83. Maruthupandian A. and Mohan V.R. (2011)., GC-MS analysis of some bioactive constituents of PterocarpusmarsupiumRoxb., International Journal of ChemTech Research, 3(3), 1652-1657.