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Assessment of Bacteria and Fungi in air from College of Applied Medical Sciences (Male) at AD-Dawadmi, Saudi Arabia

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Author Affiliations

  • 1College of Applied Medical Sciences, Shaqra University, Al-Dawadmi, KINGDOM OF SAUDI ARABIA

Int. Res. J. Biological Sci., Volume 4, Issue (9), Pages 48-53, September,10 (2015)

Abstract

This study was conducted to learn the microbial diversity in our college environment so that we can we can develop a database of the microbial load and to have a preliminary idea of the environmental status. A total of 125 samples were collected in duplicate by open plate method from 5 different sites (Microbiology Laboratory, Classrooms, Toilets, Lift and Office rooms) at College of Applied Medical Sciences, Dawadmi was experimentally investigated during Months of October 2014 to February 2015. The number of Bacteria and fungi present in our college environment were studied by exposing Nutrient agar and Sabouraud Dextrose Agar plates for 30 minutes at different sites. The bacteria isolated were; Staphylococcus aureus, Coagulase –ve Staphylococcus, Micrococcus species and Bacillus species. The moulds isolated were Aspergillus fumigatus, Aspergillus niger, Alternaria species and Penicillium species. The yeast belonging to Rhodotorula species was also isolated. The data collected in this study can used for further research. Although microorganisms are present in our college, the amount is within the range (61 - 460 CFU/m3) which indicates a low level of contamination according to the guidelines by World Health Organization.

References

  1. Slawomira M., Duma\na and Marzenna R. Dudzinska., Microbiological Indoor Air Quality in Polish Schools, Annual set the environmental protection, 15, 231-244 (2013)
  2. Huttunen K., Rintala H., Hirvonen M.R., Vepsalainen A., Hyvarinen A. and Meklin T., Indoor air particles and bioaerosols before and after renovation of moisturedamaged buildings, Environmental Research, 107, 291–298 (2008)
  3. Kim K.Y. and Kim C.N., Airbornemicrobiological characteristics in public buildings of Korea, Building and Environment, 42, 2188–2196 (2007)
  4. Stanley N.J., Kuehn T.H., Kim S.W., Raynor P.C., Anantharaman S. and Ramakrishnan M.A., Background culturable bacteria aerosol in two large public buildings using HVAC filters as long term, passive, high-volume air samplers, Journal of Environmental Monitoring, 10,474–481 (2008)
  5. Stryjakowska Sekulska M., Piotraszewska Pajak A., Szyszka A., Nowicki M and Filipiak M., Microbiological quality of Indoor air in University rooms, Polish. J. of Environ. Stud, 16(2), 623-632 (2007)
  6. Bush R.K. and Portnoy J.M., The role and abatement of fungal allergens in allergic diseases, Journal of Allergy and Clinical Immunology, 107, 430–440 (2001)
  7. Bornehag C.G., Blomquist G., Gyntelberg F., Jarvholm B., Malmberg P., Nordvall L. et al., Dampness in buildings and health, (NORDDAMP), Indoor Air,11, 72–86 (2001)
  8. Augustowska M. and Dutkiewicz J., Variability of airborne microflora in a hospital ward within aperiod of one year, Ann. Agric. Environ. Med., 13, 99-106 (2006)
  9. Atlas R. and Bartha R., Ecologia microbiana y microbiologia ambiental, Ed. Pearson Educacion, Madrid (2002)
  10. Pasquarella C., Pitzurra O. and Savino A., The index of microbial air contamination, J. Hosp. Infect., 46, 241-256 (2000)
  11. Cheesbrough M., District laboratory practice in Tropical countries, Part 2 Cambridge Low price Editions, 3-8 (2000)
  12. Zafar S., Aqil F and Ahmad I., Metal tolerance and biosorption potential of filamentous fungi isolated from metal contaminated agriculture soil, Bio. resour. Technol, 98, 2557-2561 (2007)
  13. Mostafa M.A., Al-Fifi Z.I., Alawlaqi M.M. and Al Abboud M.A., Indoor Airborne Fungi in Faculty of Science in Aboarish , Jazan University, Saudi Arabia, Journal of Jazan University, 1(2), 26-35 (2012)
  14. Shelton B.G., Kimberly H., Kirkland W., Flanders D. and Morris G.K., Profiles of airborne fungi in buildings and outdoor environments in the United States, Applied Environmental Microbiology, 68, 1743–1753 (2002)
  15. Kavita Naruka and Jyothi Gaur V., Microbial air contamination in a school, In. J. Curr. Microbiol. App. Sci, 2(12), 404-410 (2013)
  16. Geller R.J., Rubin I.L., Nodvin J.T., Teague W.G. and Frumkin H., Safe and healthy school environments, Pediatr. Clin. North. Am, 54, 351-373 (2007)
  17. Jain A.K., Survey of bioaerosol in different indoor working environments in central India, Aerobiologia, 16,221 – 225 (2000)
  18. World Health Organization: WHO indoor air quality guidelines, Copenhagen: WHO Regional Office for Europe, 1-454 (2010)