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Determination of LC50 of Lead Nitrate for a fish, Labeo rohita (Hamilton-Buchanan)

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Author Affiliations

  • 1Department of Zoology and Environmental Sciences, Punjabi University, Patiala-147002, Punjab, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (8), Pages 23-26, August,10 (2015)

Abstract

The contamination of water by heavy metals is a worldwide problem which is increasing day by day due to the anthropogenic activities. These heavy metals poses a serious health risk on human population and aquatic organisms. One such heavy metal is Lead. This paper emphasises on the determination of 96hr LC50 value ofLead Nitrate for the fish, Labeo rohita. The acute toxicity test was performed according to the standard methods in APHA and the value was calculated by probit analysis. The fish specimens were acclimatized in the laboratory conditions for 15 days. The stock solution of Lead Nitrate was prepared and the fish fingerlings were treated with various concentrations ranging from 1 mg/l to 50 mg/l for 96 hours. The results showed that the median lethal concentration (LC50) of Lead Nitrate for the fish, Labeo rohita is 34.20 mg/l. The susceptibility of Labeo rohita to the lethal effect of Lead Nitrate were dependent on duration as well as on concentration. The mortality of the fishes is directly proportional to the concentration. The use of Lead should be discouraged to protect valuable biodiversity.

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