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Molecular characterization of lactobacillus sp, from Indian curd and its Antagonistic effects on uropathogens of Diabetic patients

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Author Affiliations

  • 1Department of Immunology, Madurai Kamaraj University, Madurai, Tamil Nadu, INDIA
  • 2Department of Microbiology, Udaya College of Arts and Sciences, Kanniyakumari, Tamil Nadu, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (8), Pages 14-22, August,10 (2015)

Abstract

Diabetes mellitus is a metabolic disease and is associated with increased risk of certain complications with UTIs. The LABisfamous to yield antibacterial substances likebacteriocins which can inhibit the growth of a number of UTI pathogens. The fermented probiotic food (curd) samples were collected from home and supermarket in Tamil Nadu, India. Meanwhile the urine samples were collected from clinically UTI suspected diabetic patients from private hospitals in Tamil Nadu, India. The foremost aim of this study is to identify the different Lactobacillus sp., from curd and uropathogens from UTI diabetic patients by 16S rRNA gene sequencing. This study also stretched to extraction of bacteriocin from identified Lactobacillus sp., by fermentation and the study overextended to antibacterial activities of bacteriocin against on an identified uropathogens. All the isolates were identified by 16S rRNA sequencing. Antimicrobial activities of LAB bacteriocin was done against on UTI pathogens. L.acidophilus JCM1132, L. fermentam BCS25, L. plantarum ATCC8041 and L. casei XM2-1 from curd and Streptococcus sp, Staphylococcus sp, E.coli sp, Bacillus subtilis sp, and Pseudomonas sp were identified by 16S rRNA sequence. The fermentation process was optimized for getting maximum of products that gave antimicrobial activity against UTI pathogens. In this study, bacteriocins of L.acidophilus JCM1132 and L.fermentam BCS25 as a potential broad spectrum antibiotic against on an uropathogens (Streptococcus sp, Staphylococcus sp, E.coli sp, Bacillus subtilis sp, and Pseudomonas sp), but L.plantarum ATCC8041 and L. casei XM2-1 are mild antimicrobial activities on few isolates.

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