International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Protective Effect of Curcumin on Diethanolamine-Induced Toxic Effects on Human Spermatozoa: An in Vitro Study

Author Affiliations

  • 1Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad-380009, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (7), Pages 34-39, July,10 (2014)

Abstract

Curcumin is a yellow pigment from Curcuma longa which has desirable preventive or putative therapeutic properties. In this study protective effect of curcumin on diethanolamine-induced toxicity on human spermatozoa in in vitro condition was investigated. For this study samples were collected from normal healthy donors. After liquefaction, samples were used for preparation of sperm suspension to evaluate sperm motility, sperm viability and sperm morphology. Statistical analysis was performed using analysis of variance (ANOVA) followed by Tukey’s test and the level of significance was accepted with p0.05. When sperm suspension was treated with diethanolamine (300 µg/ml) it caused significant decrease in sperm motility and sperm viability as compared to control. Treatment also caused significantly increased different kinds of sperm morphological abnormalities as compared to control. Addition of different concentrations (10-40 µg/ml) of curcumin to sperm suspension along with diethanolamine caused significant increase in sperm motility and sperm viability as compared to treated which was time-dependent as well as concentration-dependent. As compared to treated, concentration-dependent decrease in various kinds of morphological abnormalities were also observed. This findings clearly indicate that curcumin ameliorates diethanolamine-induced spermatotoxic effect on human spermatozoa.

References

  1. Wagner P., Reassessment of Diethanolamine. (CAS Reg. No.111-42-2) United States Environmental Protection Agency, Washington, D.C. 20460.July 31 (2006)
  2. CIR Cosmetic ingredients review, Final report on the safety assessment of triethanolamine, diethanolamine, and monoethanolamine, J. Am. Coll. Toxicol,2, 183–235
  3. CIR Cosmetic ingredients review. Final report on the safety assessment of cocamide DEA, lauramide DEA, linoleamiede DEA, and oleamide DEA, J. Am. Coll. Toxicol,5, 415–454 (1986)
  4. Soreat S. A., Stabilizing acetylsalicylic acid and its salts in solution, Fr. Demande,, 143, 609 (1973)
  5. Technology Planning and Management Corporation (TPMC), Report on carcinogens Background Document for Diethanolamine, In National Toxicology Program NIEHS, Durham, NC, NO1ES85421, P. 229 (2002)
  6. Hoffmann D., Brunnemann K.D., Rivenson A. and Hecht, S.S., N nitrosiodiethanolamine: analysis, formation in tobacco products and carcinogenicity in Syrian golden hamsters, IARC Sci. Publ.,41, 299-308 (1982)
  7. Barbee S. J., and Hartung R., The effect of diethanolamine on hepatic and renal phospholipid metabolism in the rat, Toxicol. Appl. Pharmacol.,47, 421–430 (1979)
  8. Niculescu M., Wu R.,Guo Z., Costa K. and Zeisel S., Diethanolamine alters proliferation and choline metabolism in mouse neural precursor cells, Toxicol Sci.,96(2), 321-326 (2007)
  9. Bachman A.N., Kamendulis L.M. and Goodman J.I., Diethanolamine and phenobarbital produce an altered pattern of methylation in GC-rich regions of DNA in B6C3F1 mouse hepatocytes similar to that resulting from choline deficiency, Toxicol. Sci., 90, 317–325 (2006)
  10. EI. Mehallawi E. H., EI-Bassyoni A.M. and EI-Domiaty M.A., Effect of Dermal Exposure of diethanolamine on the Testes and Sperms of Adult Albino Rats, Tanta Medical Sciences Journal, 2(1), 271-285 (2007)
  11. National Toxicological Programm {NTP}, NTP technical report on the toxicity Studies of Diethanolamine (CAS No 111-42-2) administered topically and in drinking water to F344/ N Rats and B6C3F1 mice, Toxic Report Series., 20, 1-D10 (1992)
  12. Kamboj V.P., Herbal Medicine, Current Science, 78(1), 35-9 (2000)
  13. Gupta L.M. and Raina R., Side effects of some medicinal plants, Current Science, 75, 897-900 (1998)
  14. Beevers S. and Huang S., Pharmacological and clinical properties of curcumin, Botanics: Targets and Therapy, 5-18 (2011)
  15. Satoskar R.R., Shah S.J. and Shenoy S.G., Evaluation of anti-inflammatory property of curcumin (diferuloyl methane) in patients with postoperative inflammation, Int. J. Clin. Pharmcol. Ther. Toxicol., 24, 651-654 (1986)
  16. Srivastava R., Puri V., Srimal R.C. and Dhawan B.N., Effect of curcumin on platelet aggregation and vascular prostacyclin synthesis, Arzneim.-Forsch./Drug Res., 36, 715-717 (1986)
  17. Masuda T., Hidaka K., Shinohara A., Maekawa T., Takeda Y. and Yamaguchi H.J., Chemical studies on antioxidant mechanism of curcuminoid: analysis of radical reaction products from curcumin, Agric, Food Chem.,47, 71-77 (1999)
  18. Mariadason J.M., Corner G.A. and Augenlicht L.H., Genetic reprogramming in pathways of colonic cell maturationinduced by short chain fatty acids: comparison with trichostatin a, sulindac, and curcumin and implications forchemoprevention of colon cancer, Cancer, Res., 60, 4561-4572 (2000)
  19. Chan M.M., Inhibition of tumor necrosis factor by curcumin, a phytochemical, Biochemical. Pharmacol., 49, 1551-1556 (1995)
  20. Nagabhushan M., Amonkar A.J. and Bhide S.V., In vitro antimutagenicity of curcumin against environmental mutagens, Food Chem. Toxicol.,25, 545-547 (1987)
  21. Jordan W.C. and Drew C.R., Curcumin - a natural herb with anti- HIV activity, Natl. Med. Assoc.,88, 333 (1996)
  22. Khanna N.M., Turmeric - Nature’s precious gift, Curr Sci.,76(10), 1351-1356 (1999)
  23. Mathuria, N., and Verma, R. J., Curcumin ameliorates aflatoxininduced lipid peroxidation in liver, kidney and testis of mice—an in vitro study, Acta Pol Pharm,64, 413–16 (2007)
  24. Mathuria, N. and Verma, R. J., Curcumin ameliorates aflatoxininduced toxicity in mice spermatozoa, FertilSteril, 90, 775–80 (2008)
  25. Devi R., Mosheraju M. and Reddy D., Curcumin prevents chromium induced sperm characteristics in mice, IOSR Journal of Pharmacy, 2(2), 312-316 (2012)
  26. Mathuria N and Verma R. J., In vitro study on the effect of aflatoxin on human spermatozoa, ActaToxicologica, 15(1), 49-54 (2007)
  27. Panchal S. R. and Verma R. J., Spermatotoxic effect of diethanolamine: An in vitro study, Asian Pac J Reprod.,2(3), 196-200 (2013)
  28. Prasad M.R.N., Chinoy N.J. and Kadam K.M., Changes in succinic dehydrogenase levels in rat epididymis under normal and altered physiological conditions, Fertil. Steril.,23, 186-190 (1972)
  29. Gupta A. and Sarkar M., A study of adverse effect of arsenic on sperm structure and function in swiss mice, Indian J. Environ. & Ecoplan, 17(3), 445-449 (2010)
  30. Methews J. M., Garner C. E. and Matthews H. B., Metabolism, bioaccumulation and incorporation of diethanolamine into phospholipids, Chemical Research Toxicology,, 625-633 (1995)
  31. Barbee S. J. and Hartung R., Diethanolamine induced alteration of hepatic mitochondrial function and structure, Toxicol. Appl. Pharmacol.,47, 431–440 (1979)
  32. Lehman- McKeeman L. D. and Gamsky, E. A., Diethanolamine inhibits choline uptake and phosphatidylcholine synthesis in Chinese hamster ovary cells, Biochemical Biophysics Research Communication, 262, 600-604 (1999)
  33. Lehman- McKeeman L. D. and Gamsky E. A., Choline supplementation inhibits Diethanolamine induced morphological transformation in Syrian hamster embryo cells: Evidence for a carcinogenic mechanism Toxicological Sciences, 55, 303-310 (2000)
  34. Floyd R. A , Kotake Y., Hensley K., Nakae D. and Konishi Y., Reactive oxygen species in choline deficiency induced carcinogenesis and nitrone inhibition, Mol. Cell. Biochem.,234-235(1-2), 195-203 (2002)
  35. Rahimtula A.D., Bereziat J.C., Bussacchini-Griot V. and Bartsch H., Lipid peroxidation as a possible cause of ochratoxinA toxicity, Biochem. Pharmacol.,37, 4469 (1988)
  36. Makkar k., Agarwal A. and Sharma R., Oxidative stress and male infertility, India, J Med Res,139, 375-367 (2009)
  37. Agarwal A., Prabakaran S.A. and Said T.M.,– Prevention of oxidative stress injury to sperm, Journal of Andrology, 26, 654-660 (2005)
  38. Masuda T., Maekawa T., Hidaka K., Bando H., TakedaY.and Yamaguchi H., Chemical studies on antioxidant mechanisms of curcumin: analysis of oxidative coupling products from curcumin and linoleate, J Agric Food Chem,49, 2539-2547(2001)
  39. Chan W. and Wu H., Protective effects of curcumin on methylglyoxal-induced oxidative DNA damage and cell injury in human mononuclear cells, Acta Pharmacologica Sinica, 27(9), 1192-1198 (2006)