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Biochemical factors, MDA levels and Antioxidant activity in Opium addicted Hamsters

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Author Affiliations

  • 1Department of Biochemistry and Nutrition, Hamadan University of Medical Sciences, Hamadan, IRAN
  • 2 Research Center for Molecular Medicine, Hamadan University of and Medical Sciences, Hamadan, IRAN
  • 3 Department of Laboratory Sciences, Para-medical Faculty, University of Medical science, Kermanshah, IRAN
  • 4 Kermanshah University of Medical Sciences, Kermanshah, IRAN
  • 5 Department of Health, University of Medical science, Kermanshah, IRAN
  • 6 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IRAN
  • 7 Department of Biology, Islamic Azad University, Borojerd Branch, Borojerd, IRAN
  • 8 Student Research Committee, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IRAN
  • 9 Sabzevar University of Medical Sciences, Sabzevar, IRAN

Int. Res. J. Biological Sci., Volume 3, Issue (2), Pages 21-24, February,10 (2014)

Abstract

In this experiment, 12 male golden Syrian hamsters were divided into two groups containing six animalseach: Group 1 received chow diet (control); group 2 received opium (addicted). All hamsters were sacrificed after 24 hours of the final treatment (after 4four weeks), and the blood of animals were collected. Lipid profiles and liver enzymes were measured enzymatically. Atherogenic Index (AI) and LDL-C were calculated. Also, the LDL-C to HDL-C ratiowas determined.Serum super oxide dismutase (SOD) activity was measured by the method Misra and Fridovich method. The level of malondialdehyde (MDA) was evaluated by Ohkawa et al method. Catalase (CAT) and reduced glutathione (GSH) activity were measured by Aebi and Beutler E, et al. methods respectively. Amount of total cholesterol had a non-significant decreased in opium group. Serum TG and VLDL-C increased in opium group but it was not significant. Change in liver enzymes, were not significant in opium group compared to control hamster. The plasma concentration of MDA markedly increased in opium (p < 0.01) group compared to control. SOD, GSH and catalase levels also markedly reduced in opium (p <0.05) group compared to healthy hamster. In conclusion, oxidative stress is increased in opium treated animals.

References

  1. Willcox B.J., Curb J.D. and Rodriguez B.L., Antioxidants in cardiovascular health and disease: key lessons from epidemiologic studies, Am J Cardiol, 101, 75–86 (2008)
  2. Sarmandal C.V., Cancer, Heart and other Chronic Diseases: Some Preventive Measures to Control Lipid Peroxidation through Choice of Edible Oils, International Research Journal of Biological Sciences, 1(6), 68-75 (2012)
  3. Elson D.F. and Meredith M., Therapy for type 2 diabetes mellitus, WMJ, 97, 49-54 (1998)
  4. Rajabizade G., Ramezani M.A. and Shakibi M.R., Prevalence of Opium Addiction in Iranian Drivers 2001-2003, J Med Sci, 210–3 (2004)
  5. Shiri R., Hassani K.F., Ansari M., Association between opium abuse and comorbidity in diabetic men, Am J Addict, 15, 468–72 (2006)
  6. Kalaiselvan A., Gokulakrishnan K., Anand T., Akhilesh U. and Velavan S, Preventive Effect of Shorea Robusta Bark Extract against Diethylnitrosamine -Induced Hepatocellular Carcinoma in Rats, International Research Journal of Medical Sciences, 1(1), 2-9 (2013)
  7. Mohammadi A., Darabi M., Nasry M., Saabet-Jahromi M.J., Malek-Pour-Afshar R. and Sheibani H., Effect of opium addiction on lipid prole and atherosclerosis formation in hypercholesterolemic rabbits, Experimental and Toxicologic Pathology, 61,145–149 (2009)
  8. Yousefi B.V., Amraeai E., Salehh H., Sadeghi L., Najafi L. and Fazilati M., Evaluation of Iron Oxide nanoparticles effects on tissue and Enzymes of Thyroid in Rats, International Research Journal of Biological Sciences, 2(7), 67-69, (2013)
  9. Husain K., Mejia J., Lalla J. and Kazim S.H., Dose response of alcohol-induced changes in BP, nitric oxide and antioxidants in rat plasma, Pharmacological Research, 51, 337–343 (2005)
  10. Mohammadi A., Abbasi Oshaghi E., Noori Sorkhani A., Oubari F., HosseiniKia R. and Rezaei A., Effect of Opium on Lipid Profile and Expression of Liver X Receptor Alpha (LXR) in Normolipidemic Mouse, Food and Nutrition Sciences, 3(2), 249-254 (2012)
  11. Abbasi Oshaghi E., Sorkhani A.N. and Rezaei A., Effects of Walnut on Lipid Profile as Well as the Expression of Sterol-Regulatory Element Binding Protein-1c(SREBP-1c) and Peroxisome Proliferator Activated Receptors (PPAR) in Diabetic Rat, Food and Nutrition Sciences, 3, 255-259 (2012)
  12. Misra H.P. and Fridovich I., The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide-dismutase, J BiolChem, 247, 3170–5 (1972)
  13. BeutlerE., Duron O. and Kelly B.M., Improved method for the determination of blood glutathione, J Lab Clin Med, 61, 882-8 (1963)
  14. Vasdev S., Gill V. and Singal P.K., Beneficial effect of low ethanol intake on the cardiovascular system: possible biochemical mechanisms,Vascular Health and Risk Management, 2(3), 263–276 (2006)
  15. Schwenke D.C., Antioxidants and atherogenesis, Biochem. J Nutr9, 424-445 (1998)
  16. Lonn M.E., Dennis J.M. and Stocker R.,Actions of ‘‘antioxidants’’ in the protection against atherosclerosis, Free Radical Biology and Medicine, 15; 53(4), 863-84 (2012)
  17. Indumati V., Patil V.S., Krishnaswamy D., Satishkumar D., Vijay V., Mahesh S. and Rajeshwari V., Non-Hdl cholesterol and Ldl-C/Hdl-C ratio in type II diabetic patients, International Journal of Pharma and Bio Sciences, B71-77 (2011)
  18. Omenn G. S., Goodman G. E., Thornquist M. D., Balmes J., Cullen M.R. and Glass A., Effects of a combination of beta carotene and vitamin A on lung cancer and cardiovascular disease, N. Engl. J. Med,334, 1150–1155 (1996)
  19. Sugamura k. and Keaney J.F., Reactive oxygen species in cardiovascular disease, Free Radical Biology & Medicine, 51, 978–992 (2011)
  20. Sirappuselvi S. and Chitra M., In vitro Antioxidant Activity of Cassia tora Lin, International Research Journal of Biological Sciences,1(6), 57-61 (2012)
  21. Aweng E.R., Hanisah N., Mohd Nawi M.A.,, Nurhanan Murni Y. and Shamsul M., Antioxidant Activity and Phenolic Compounds of Vitex Trifolia Var, Simplicifolia Associated with Anticancer, International Research Journal of Biological Sciences, 1(3), 65-68, (2012)
  22. Lowe H.I., Watson C.T., Badal S., Ateh E.N., Toyang N.J. and Bryant J., Anti-angiogenic properties of the Jamaican ball moss, (Tillandsia recurvata L.), International Research Journal of Biological Sciences, 1(4), 73-76 (2012)
  23. Rahman k., AlamD.M. and Islam N., Some Physical and Mechanical Properties of Bamboo Mat-Wood Veneer Plywood, International Research Journal of Biological Sciences, 1(2), 61-64 (2012)
  24. Alam E.A., Initiation of Pharmaceutical Factories depending on more Application of Biotechnology on some Medicinal Plants Review Article (In Vitro Production of some Antioxidant, Analgesic, Antibacterial, Antidiabetic agent), Res J Recent Sci.,1(ISC-2011) ,398-404 (2012)
  25. Patil Sunil J. and Patil H.M., Ethnomedicinal Herbal Recipes from Satpura Hill Ranges of ShirpurTahsil, Dhule, Maharashtra, India, Res. J. Recent Sci., 1(ISC-2011), 333-366 (2012)
  26. Sumanth M. andRana A.C., In vivo antioxidant activity of hydroalcoholic extract of Taraxacusofficinale in rats.Indian J Pharmacology, 38(1): 54-55 (2006)
  27. Pratico D.,Lipid Peroxidation in Mouse Models of Atherosclerosis, Trends Cardiovasc Med,11,112–116 2001) 28.Napoli C. and Ignarro L.J., Nitric oxide and atherosclerosis Nitric oxide: Biology and Chemistry, 5(2), 88–97 (2001)
  28. Napoli C. and Ignarro L.J., Nitric oxide and atherosclerosis Nitric oxide: Biology and Chemistry, 5(2), 88–97 (2001)
  29. Madhavan V, Shah P., Murali A. and Yoganarasimhan S.N., In vitro and in vivo antioxidant activity studies on the roots of Toddaliaasiatica (L.) Lam. (Rutaceae).Asian Journal of Traditional Medicines, 5 (5), 188-198 (2010)