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Genomic Discrimination of Eleven Commercial Mushrooms by DNA Fingerprinting using RAPD Marker

Author Affiliations

  • 1Sangenomics Research Labs, 16, 2nd Cross, Krishna Reddy Colony, Domlur Layout, Airport Road, Bangalore- 560071, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 1-5, October,10 (2013)


In today’s world with burgeoning population the growth cultivation of edible mushrooms holds great significance because of their nutritive and therapeutic properties. The advances in research on mushroom breeding and production are highly constrained compared to other crops; due to lack of previous knowledge in mushroom genetic. Present study focuses on establishing phylogenetic relationship among the eleven elite edible mushrooms using RAPD markers. Eleven mushroom samples namely 1) A. bisporus (Button), 2) A. bisporus (Portobello), 3) P. eryngii (King Oyster), 4) L. edodes (shiitake), 5) H. tessellatus (Brown Shimeji), 6) H. tessellatus (White Shimeji), 7) F. velutipes (Enoki), 8) P. ostreatus (Oyster), 9) P. djamor (Pink Oyster), 10) C. indica (Milky), and 11) P. florida (Florida Oyster) were used. Molecular markers serve as quick and reliable tool to establish the identities of mushrooms and are helpful in mushroom taxonomy. Among the various primers used to OPZ 10 gave the most distinguished and scorable band pattern. To estimate the similarity and genetic distance among different mushroom, cluster analysis based on frequency similarity was performed. The RAPD analysis in this study has proven to be useful in discrimination, characterization and differentiation of the fungal cultivars.


  1. Iwalokun B.A., Usen U.A., Otunba A.A. and Olukoya D.K., Comparative phytochemical evaluation, antimicrobial and antioxidant properties of Pleurotus ostreatus, AJB, 6 (15), 1732-1739 (2007)
  2. Sadler M., Nutritional properties of edible fungi, Br. Nutr. Found. Nutr. Bull., 28, 305-308 (2003)
  3. Khan S.M., Nawaz A., Malik W., Javed N., Yasmin T., Rehman M.U., Qayyum A., Iqbal Q., Ahmad T. and Khan A.A., Morphological and molecular characterization of Oyster mushroom (Pleurotus spp.), AJB, 10(14), 2638-2643 (2011)
  4. Sharma N., Medicinal uses of macrofungi, Ethnobotan, 15, 97-99 (2003)
  5. Çaglarirmak N., The nutrients of exotic mushrooms (Lentinulaedodes and Pleurotusspecies) and an estimated approach to the volatile compounds, Food Chem., (105), 1188–1194 (2007)
  6. Elmastas M., Isildak O., Turkekul I. and Temur N., Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms, J. Food Comp. Anal., 20, 337–345 (2007)
  7. Lindequist U., Niedermeyer T.H.J. and Julich W.D., The pharmacological potential of mushrooms, eCAM, 2(3), 285-299 (2005)
  8. Hadar Y., Keren Z., Gorodecki B., and Ardon O., Utilization of lignocellulosic waste by the edible mushroom Pleurotus. Biodegradation,189-205 (1992)
  9. Jaradat A.A., Genetic resources of energy crops: biological systems to combat climate change, AJCS, 309-323 (2010)
  10. Jose N. and Janardhanan K.K. Antioxidant and antitumor activity of Pleurotus florida, Curr Sci, 79(7), 941-943 (2000)
  11. Yang J.H., Lin H.C. and Mau J.L. Antioxidant properties of several commercial mushrooms, Food Chem., 77, 229-235 (2002)
  12. Ribeiro B., Rangel J., Valenta’o P., Baptista P., Seabra R. M. and Andrade P. B., Contents of carboxylic acids and two phenolics and antioxidant activity of dried portuguese wild edible mushrooms, J. Agric. Food Chem., 54, 8530–8537 (2006)
  13. Chandra S., Ghosh K. and Acharya K. Comparative studies on the Indian cultivated Pleurotus species by RAPD fingerprinting. Nature and Science, 8(7), 90-94 (2010)
  14. Williams J.G., Kubelik A.R., Livak K.J., RafalskiJ.A. and Tingey S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18 (22), 6531-6535 (1990)
  15. Ravash, R., Shiran, B., Alavi, A. and Zarvagis, J. Evaluation of genetic diversity in Oyster mushroom (Pleurotus eryngii) isolates using RAPD marker, J. Sci. & Technol. Agric. & Natur. Resour., 13, 739-741 (2009)
  16. Staniaszek M., Marczewski W., Szudyga K., Maszkiewicz J., Czaplicki A. and Qian G. Genetic relationship between Polish and Chinese samples of the mushroom Agaricus bisporus (Lange) Sing., determined by the RAPD method, J. Appl. Genet., 43, 43-47 (2002)
  17. Stajic M., Sikorski J., Wasser S.P. and Nevo E., Genetic similarity and taxonomic relationships within the genus Pleurotus (higher Basidiomycetes) determined by RAPD analysis, Mycotoxon, 93, 247-255 (2005)
  18. Pei-Sheng Y., Luo X.C. and Zhou Q., RFLP analysis of amplified nuclear ribosomal DNA in the genus Auricularia, Myco system, 18, 206–213 (1999)
  19. Magdum S.S., A Reliable and High Yielding Method for Isolation of Genomic DNA from Ammi majus, Int. Res. J. Biological Sci., 2(1,), 57-60 (2013)