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Urease Inhibitory activity of fractions from Harungana madagascariensis fruit Lam. Ex Poir.(Hyperiaceae)

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Author Affiliations

  • 1Department of Pharmacognosy and Traditional Medicine, Faculty of Pharmaceutical Sciences, Chukwuemeka Odumegwu Ojukwu Unuversity, Igbariam Campus and Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria
  • 2Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria and Nuclei for Phytomedicines and Chemical Ecology, Central Research Laboratory for Phytomedicines, Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria
  • 3Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria

Int. Res. J. Biological Sci., Volume 12, Issue (1), Pages 16-20, May,10 (2023)

Abstract

Harungana madagascariensis is used in ethnomedicine to treat stomach diseases, bacterial infections some of which are due to ureolytic pathogens, and anemia among others. This study is a urease inhibition activity guided fractionation of the crude ethanol extract of the fruits of Harungana madagascariensis. The fruit of H. madagascariensis was extracted with 70% aqueous ethanol by cold maceration. The crude aqueous ethanol extract (CEE) was defatted with n-hexane and further partitioned with ethyl acetate to give n-hexane portion (HSF), ethyl acetate portion (ESF) and aqueous portion (ASF). Fractionation of most active ESF was done using chromatographic techniques and it yielded four fractions EA1 –EA4. The modified Berthelot’s colorimetric method was used for the in vitro urease inhibition assay. Standard phytochemical methods were used for phytochemical screening. The trend of percentage (%) inhibition for the crude extract and its partitioned fractions was: CEE(35.03±0.05)>ESF(33.29±0.00)>ASF(17.10±0.06)>HSF(12.97±0.06) at 2.00 mg/ml while the trend of activity for the chromatography fractions from the most active ESF at 2.00 mg/ml was observed thus; EA3(60.74±0.00) > EA4 (26.69± 0.06)>EA2(16.79±0.06)>EA1(14.16±0.06). The most active chromatography fraction EA3 had an IC50 of 1.0 mg/ml. phytochemical investigation of EA3 revealed the presence of anthraquinones and phenolics. This supports the use in ethnomedicine practice, of H. madagascariensis to treat various stomach and urinary diseases and also a potential lead source of urease inhibitors for sustainable agronomy.

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