International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Decolorization of Reactive Blue MR, using Aspergillus species Isolated from Textile Waste Water

Author Affiliations

  • 1Department of Biotechnology, Chaudhary Devi Lal University, Sirsa, INDIA
  • 2 Department of Energy and Environmental Sciences, Chaudhary Devi Lal University, Sirsa, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (1), Pages 24-29, May,10 (2012)

Abstract

The present investigation focused on the isolation and characterization of fungal strains, which can efficiently decolorize the textile dye, reactive Blue MR. A total of five indigenous fungal strains were isolated from the effluents collected around the discharge site of textile industry situated in Panipat. Effluent samples were also analyzed for their physiochemical properties. Aspergillus allhabadii, A. niger and A. sulphureus were successfully identified using macroscopic and microscopic study referring relevant literature. Decolorization capabilities of these fungal species were evaluated for reactive blue MR dye (100-300mg/L) in carbon limited Czapek Dox broth (0.5%), carried out under static in vitro condition. It was found that A. allhabadii and A. sulphureus showed higher decolorization capabilities (95.13±0.11%), (93.01±0.25%) with 200mg/L dye, but A. niger showed higher decolorization (83.14±0.19%) with 100 mg/L after ten days of incubation. Decolorization efficiency was also investigated in different carbon sources and found sucrose was the best carbon for all the fungal strains. The fungal isolates were found efficient in decolorization, which proves that these indigenous fungi are potential candidates for bioremediation.

References

  1. Zollinger H., Color Chemistry: Syntheses, Properties and Applications of Organic Dyes and Pigments (2nd Edn), VCH Publications, New York, 496 (1991)
  2. Campos R., Kandelbauer A., Robra K.H., Artur C.P. and Gubitz G.M., Indigo degradation with purified laccases from Trametes hirsuta and Sclerotim rolfsii, J. Biotechnol., 8, 131-139 (2001)
  3. McMullan G., Robinson T., Marchant R. and Nigam P.,Remediation of dyes in textile effluent: a critical review on current treatment technologies with proposed alternatives, Biosource Technol., 77, 247-255 (2001)
  4. Chung K.T. and Stevens S.E., Decolorization of azo dyes by environmental microorganisms and helminthes, Environ. Toxicol. Chem., 12, 2121-2132 (1993)
  5. Shaul G.M., Holdaworth T.J., Dempsey C.R. and Dostal K.A., Fate of water soluble azo dyes in the activated sludge process, Chemosphere, 22, 107-119 (1991)
  6. Kirk T.K., Lamar R.T. and Glaser J.A., The Potential of White rot Fungi in Bioremediation. Biotechnology and Environmental Science Molecular Approaches, Mongkolsuk, S. (Ed.), Plenum Press, UK, 131-138 (1992)
  7. Koichi H., Yoshio W. and Kazunori N., Decolorization of azo dye by the white-rot basidiomycetes Phanerochaete sordida and by its manganese peroxidase, J. Biosci. Bioengineer, 95(5), 455-459 (2003)
  8. Sharma P., Singh L. and Dilbaghi N., Optimization of process variables for decolorization of Disperse Yellow 211 by Bacillus subtilis using Box- Behnken design, J. Haz. Matter., 164, 1024-1029 (2009)
  9. Rodriguez E., Pickard M.A. and Rafael Vazquez-Duhalt, Industrial dye decolorization by laccases from ligniolytic fungi, Curr.Microbiol., 38, 27-32 (1999)
  10. Murugesan K., Nam I., Kim Y. and Chang Y., Decolorization of reactive dyes by a thermostable laccase produced by Ganoderma lucidum in solid state culture, Enzyme Microbi. Technol., 40, 1662-1672 (2007)
  11. Clesceri L.S., Greenburg A.E. and Trussell R.R., Standard methods for the examination of water and wastewater (17th Edn), American public Health Association, Washington, DC., USA (1989)
  12. Gilman J.C., A manual of soil fungi (Revised 2nd Edn), Oxford & IBH publishing Co. (1944)
  13. Barnett H.L. and Hunter B.B., Illustrated genera of Imperfect Fungi, Burgress Publishing Company, Minneapolis, Minnesota (1972)
  14. Ellis M.B., Dermatacious Hyphomycetes, Commonwealth Mycological Institute, Kew, Surrey, UK (1976)
  15. Domsch K.H., Gams W. and Anderson T.H., Compendium of soil fungi, Academic press. A subsidiary of Harcourt Brace Jovanovich, publisher, (1980)
  16. Olukanni O.D., Osuntoki A.A. and Gbenle G.O., Textile effluent biodegradation potentials of textile adapted and non-adapted bacteria, African J. Biotechnol., 5, 1980-1984 (2006)
  17. Devi M. and Kaushik B.D., Decolorization of textile dyes and dye effluent by Aspergillus Spp., Indian J. Microbiol., 45, 41-44 (2005)
  18. Wood W.A. and Kellogg S.T., Biomass, cellulose and hemicelluloses, Methods Enzymol., 160, 632-634 (1988)
  19. Buckley C.A., Membrane technology for the treatment of dye house effluents, Water Sci. Techno., 25 (10), 203-209 (1992)
  20. Vandevivre P.C., Bianchi R. and Verstraete W., Treatment and reuse of wastewater from the textile wet-processing industry: review of emerging technologies, J. Chem.Technol.Biotechnol., 72, 289- 302 (1998)
  21. Ponraj M., Gokila K. and Vasudeo Z., Bacterial decolorization of textile dye- Orange 3R, International J. Adv. Biotechnol. Res., 2(1), 168-177 (2011)
  22. Selvam K., Swaminathan K. and Chae K.S., Decolourization of azo dyes and a dye industry effluent by a white rot fungus Thelephora sp. Bioresour Technol., 88, 115-119 (2003)
  23. Wesenberg D., Kyriakides I. and Agathos S.N., White-rot fungi and their enzymes for the treatment of industrial dye effluents, Biotechnol. Adv., 22, 281-287 (2003)
  24. Fu Y. and Viraraghvan T., Dye biosorption sites in Aspergillus niger, Biores. Technol., 82, 139-145, (2002)
  25. Knapp J.S., Newby P.S. and Reece L.P., Decolorization of dyes by wood rotting basidiomycete fungi, Enzyme Microbiol. Technol., 17, 664-668 (1995)
  26. Swamy, J. and Ramsay J.A., The evaluation of white rot fungi in the decoloration of textile dyes, Enzyme Microbiol. Technol., 24, 130-137 (1999)
  27. Zhang, F.M., Knapp J.S. and Tapley K.N., Decolourization of cotton bleaching effluent with wood rotting fungus, Water Res., 33, 919-928 (1999)
  28. Ali N., Ikramullah L.G., Hameed A. and Ahmed S., Decolorization of Acid red 151 by Aspergillus niger SA1 under different physicochemical conditions, World J. Microbiol., 24, 1099-1105 (2008)
  29. Sumathi S. and Manju B.S., Uptake of reactive Textile dyes by Aspergillus foetidus. Enzyme Microb. Technol., 27(6), 347-355 (2000)
  30. Aksu Z. and Tezer S., Equilibrium and kinetic modelling of biosorption of Remazol Black B by Rhizopus arrhizus in a batch system: effect of temperature, Process Biochem., 36, 431-439 (2000)
  31. Mansur M., Arias M.E. and Copa Patino J.L., The white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities, Mycologia, 95(6), 1013-1020 (2003)
  32. Bhatt M., Patel M., Rawal B., Novotny C., Molitoris H.P. and Sasek V., Biological decolorization of synthetic dye RBBR in contaminated soil, W. J. Microbiol. Biotechnol., 16, 195-198 (2000)
  33. Pearce C.I., Lloyd J.R. and Guthrie J.T., The removal of colour from textile wastewater using whole bacterial cells: a review, Dyes Pigments, 58, 179-196 (2003)
  34. Hu T.L. and Wu S.C., Assessment of the effect of azo dye Rp2B on the growth of nitrogen fixing cyanobacterium - Anabena sp., Biores. Technol., 77, 3-95 (2001)