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Establishment of maternity from sternum bone of an unidentified dead body - A case study

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Author Affiliations

  • 1DNA Division, Regional Forensic Science Laboratory, Central Range, Mandi - 175002, Himachal Pradesh, India
  • 2Directorate of Forensics Services, Junga, Shimla - 171218, Himachal Pradesh, India

Res. J. Forensic Sci., Volume 9, Issue (1), Pages 7-10, January,29 (2021)

Abstract

Sternum bone is one of the most common exhibits received in forensic laboratories from unidentified dead bodies. These bones are received for identification of dead bodies or establishment of maternity and paternity. Sternum bones are a good source for DNA profiling as they contain abundant cells. The costal cartilage tissue attached with sternum bone act as a source of clean DNA profile. In the present study, maternity was established from sternum bone of an unidentified dead body. DNA from sternum bone was isolated using magnetic bead based method with Qiagen EZ1 Advanced XL BioRobot, whereas DNA from FTA card of putative son was purified using FTA purification buffer reagent. The isolated and purified DNA was subjected to Multiplex PCR amplification using PowerPlex® 21 System kit (Promega Corporation, Wisconsin, United States). Capillary electrophoresis of amplified products was done with 3130 Genetic Analyzer (Applied Biosystems, U.S.A.). The data were analyzed using GeneMapper®ID Software Version 3.2. The autosomal short tandem repeats (STR) DNA analysis confirmed the maternity of unidentified dead bodies. Hence, human sternum bones are a good source of DNA for establishment of identity, maternity and paternity.

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