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Species level identification of Lepus nigricollis from forensic sample using molecular marker and trichology: (First Report)

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Author Affiliations

  • 1Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, 444602, India
  • 2State Forensic Science Laboratory, Amravati, 444602, India
  • 3Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, 444602, India

Res. J. Forensic Sci., Volume 9, Issue (1), Pages 1-6, January,29 (2021)

Abstract

The application of wild life forensics involves identification of species based on the similarities in the DNA sequences of the selective loci. In case of illegal poaching the evidence available are pieces of meat, bones, hairs, blood strains, skin, etc. In the present study pouched samples (meat, hairs with follicle, blood stain and clot) were seized by forest officials but the exact species could not be identified by phenotypic methods. Nucleotide sequence of Cytochrome b (cytb) were 100% similar of all the four seized samples. This revealed that the samples were from same species. The sequences were found to have >90% similarity with other species of Lepus. The exact species could not be identified using Blast analysis but revealed that samples belonged to genus Lepus. Trichological analysis of the seized hairs and comparison with known species revealed that the seized samples belonged to species Lepus nigricollis also called as Indian Hare.

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