The effects of heat and molotov incendiary device fluids on DNA analysis
- 1Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
- 2Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
- 3Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
- 4Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
- 5Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
- 6Faculty of Pharmacy, Mersin University, Mersin, Turkey
- 7Faculty of Health Sciences, Istanbul Yeni Yuzyil University, Istanbul, Turkey
- 8Department of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
- 9Faculty of Health Sciences, Istanbul Yeni Yuzyil University, Istanbul, Turkey
Res. J. Forensic Sci., Volume 7, Issue (2), Pages 8-14, July,29 (2019)
Using the findings from terror attacks such as hand-made incendiary devices, the chemical constituency of the explosive can be elucidated to identify the terrorist group responsible for the attack. It is also possible to clarify the incident by analyzing the DNA from the fingerprint. The purpose of this study is to obtain DNA from the fingerprint found on the explosive at the crime scene and to analyze the thermal effect of the DNA on profiling. Fingerprint samples were put on glass, paper and metal sheet surfaces and were individually exposed to diffrent heat conditions for 3 minutes. A total of 108 samples were subjected to DNA analysis using GlobalFiler® PCR Amplification Kit and were typed using the ABI 3130 electrophoresis device. From the samples, complete DNA profile was obtained at 50oC and 90oC on the paper surface and at 50oC on the glass and metal surfaces. On all surfaces and at all temperatures, the gender-identifying locus, amelogenin, was able to be observed. This study, which is conducted for the first time in Turkey, may significantly contribute to the identification of attackers and clarification of the events particularly in terror acts.
- Champod C., Lennard C., Margoti P. and Stoilovic M. (2004)., Fingerprints and other skin ridge impressions., CRC Press LLC, Boca Raton, 35-47. ISBN: 978-14-98728-93-95.
- Cheng C., Kirkbride T.E., Batchelder D.N., Lacey R.J. and Sheldon T.G. (1995)., In situ detection and identification of trace explosives by Raman microscopy., J. Forensic Sci., 40(1), 31-37.
- Rowell F., Seviour J., Lim A.Y., Elumbaring-Salazar C.G., Loke J. and Ma J. (2012)., Detection of nitro-organic and peroxide explosives in latent finger marks by DART- and SALDI-TOF-mass spectrometry., Forensic Sci. Int., 221(1), 84-91.
- Ostojic L. and Wurmbach E. (2017)., Analysis of fingerprint samples, testing various conditions, for forensic DNA identification., Sci. Justice, 57(1), 35-40.
- Maynard P., Jenkins P.J., Edey C., Payne G., Lennard C., McDonagh A. and Roux C. (2009)., Near infrared imaging for the improved detection of fingermarks on difficult surfaces., Austr. J. Forensic Sci., 41(1), 43-62.
- Wickenheiser R.A. (2002)., Trace DNA: Areview, discussion of theory, and application of the transfer of tracequantities of DNA through skin contact., J. Forensic Sci., 47, 442-450.
- Alessandrini F., Cecati M., Pesaresi M., Turchi C., Carle F. and Tagliabracci A. (2003)., Fingerprints as evidence for a genetic profile: morphological study on fingerprints and analysis of exogenous and individual factors affecting DNA typing., J. Forensic Sci., 48, 586-592.
- Caragine T., Mikulasovich R., Tamariz J., Bajda E., Sebestyen J., Baum H. and Prinz M. (2009)., Validation of testing and interpretation protocols for low template DNA samples using AmpFlSTR Identifiler., Croat. Med. J., 50, 250-267.
- Karakus O. and Demir S. (2005)., An alternative method for personal identification: Poroscopy., Polis Bilim. Derg., 7(4), 15-33.
- Pesaresi M., Buscemi L., Alessandrini F., Cecati M. and Tagliabracci A. (2003)., Qualitative and quantitative analysis of DNA recovered from fingerprints., Int. Cong. Series., 1239(1), 947-951.
- Deans J. (2006)., Recovery of fingerprints from fire scenes and associated evidence., Science & justice: journal of the Forensic Science Society, 46(3), 153-168.
- Zhang L. and Wu Q. (2005)., Single gene retrieval from thermally degraded DNA., J. Biosci., 30(5), 599-604.
- Horsman-Hall K.M., Orihuela Y., Karczynski S.L., Davis A.L., Ban J.D. and Greenspoon S.A. (2009)., Development of STR profiles from firearms and fired cartridge cases., Forensic Sci. Int. Genet., 3(4), 242-250.
- Shelef R., Levy A., Rhima I., Tsaroom S. and Elkayam R. (1996)., Development of Latent Fingerprints From Incendiary Bottles: Development of Latent Fingerprints from Unignited Incendiary Bottles; Optimization of Small Particle Reagent for the Development of Latent Fingerprints From Glass Surfaces Washed in Accelerant Fluids; Recovery of Latent Fingerprints From So., Journal of Forensic Identification, 46(5), 556-569.
- Kumar P., Gupta R., Singh R. and Jasuja O.P. (2015)., Effects of latent fingerprint development reagents on subsequent forensic DNA typing: A review., Journal of forensic and legal medicine, 32, 64-69.
- Gardner S.J., Cordingley T.H. and Francis S.C. (2016)., An investigation into effective methodologies for latent fingerprint enhancement on items recovered from fire., Sci. and Just., 56(4), 241-246.
- Opel K.L., Chung D.T., Drabek J., Tatarek N.E., Jantz L.M. and McCord B.R. (2006)., The application of miniplex primer sets in the analysis of degraded DNA from human skeletal remains., J. Forensic Sci., 51(2), 351-356.