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Extraction, Analysis and Quantization of Endogenous Level of GHB from Human Hair

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Author Affiliations

  • 1Anglia Ruskin University, East Road, Cambridge, CB1 1PT, ENGLAND
  • 2 Sherlock Institute of Forensic Science India, Hudson Line, Delhi 110009, INDIA

Res. J. Forensic Sci., Volume 3, Issue (3), Pages 1-6, March,29 (2015)

Abstract

Hair is a physical evidence to describe criminals. Hair plays a multi-disciplinary role, role of forensic chemist to identify drug from hair and a forensic biologist can take DNA. It can give information of drug history, identification of human or animal, mode of hair removed from skin, and DNA profile of person may be victim or criminal. According to Baumgartner et al., to collect hair sample we do not need any special equipment as well as for storage. Presence of drugs in urine and fecal analysis zero to 3 or 4 days only but for hair it is months to years. Drugs analysis of hair became very common in Forensic laboratory because a long enough hair can present long term history of drugs taking by individual. Tt is acknowledged that in common, average growth of hair in every person is 1 cm per month. So segment of hairs sample can give information of drugs taken during months of calendar year. According toKintz et al, “detection of gamma-hydroxy butyrate (GHB) in hair following an alleged drug-facilitated sexual assault (DFSA).” There are a number of cases reported which are related to Gamma Hydroxybutyrate (GHB) abuse. It is a club or date rape drug, used for sexual and other abuse. The aim of this research will be to describe what is the best technique that can be used for the analysis of hair to get endogenous level of GHB in hair? Decontamination of hair done by washing with 5 ml of methylene chloride twice at room temperature. To predict correct interpretation washing is required. To improve quality of results we operate chromatography –mass spectroscopy (GC-MS) test. It is difficult to make cross relativity between qualitative and quantitative data for immunological test to give conformity results but GC-MS present very analogous data. Kronstrand et al., These are some different standard concentrations, which were used in this laboratory work for analysis. In our experiment, we also took three different samples weighing 20mg each for analysis. Data of GC-MS results, gives pick area for the quantization ions 117(m/z), 147 (m/z), 233 (m/z), internal standard 145 (m/z) as well as the standard concentrations and our three samples. From these, we took ion 233(m/z) as Q, and together with the internal standard 145 (m/z), the table have prepared for peak area ratio of the various concentrations from by the formula 233(m/z)/IS. Ions selected for Single Ion Monitoring (SIM) of GHB were 233, 204 and 117 m/z (graph 1.0), for result of this experiment this are C=117, C=147 and Q = 233. The 5-points calibration curves were linear over the concentration range evaluated. The resultant linear regression equation extrapolated from graph 1.1 is given as (y = 0.0036x + 0.0033), where 0.0036 represents the gradient and 0.0033 being the intercept. It also has a measure of precision or the coefficient of goodness of how well experimental data is spread on the line as (R). From the literature, previous studies by were excellent (R=0.9947 for this experiment). Limit of quantisation (LOQ) was identified as the lowest calibrator point is102.5 ng/mg.Role of drug analysis from hair sample to give information of drug history and quantity of used. GC-MS technique which have big contribution now and in further development of forensic science because their aspects are endless.

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