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Microscopical analyses on characterization of peripheral blood cells of Western pond turtle (Actinemys marmorata) (Baird and Girard, 1852)

Author Affiliations

  • 1State Forensic Science Laboratory, Bhubaneswar-751010, Odisha, India

Res. J. Animal, Veterinary and Fishery Sci., Volume 9, Issue (1), Pages 25-30, May,24 (2021)

Abstract

The aim of the present study is to investigate the microscopical characterization of peripheral blood cells namely erythrocytes, and leucocytes of Western pond turtle. However, no previous report on microscopical characterization of blood cells of Western pond turtle has been reported. One turtle was taken for this study. Blood sample was collected and blood smears were immediately prepared without any anticoagulant. Then after drying in air, fixed with methanol and stained with Giemsa stain. The erythrocytes observed were mainly elliptical in shape with ellipsoid or irregularly round nuclei. Besides that different shapes of erythrocytes were also observed. Five types of leucocytes including lymphocytes, monocytes, heterophils, eosinophils and basophils are seen in the blood smears of peripheral blood. The aim of the study is to provide baseline data on the description and morphological classification of blood cells in Western pond turtles by which their physiological states can also be known and they can be better treated. The data serve as a baseline for future hematologic studies in this species.

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