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Assessment of media modification and induction of calli on coconut (Cocos nucifera L.) palm explants

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Author Affiliations

  • 1Physiology Division, Nigeria Institute for Oil Palm Research, Benin City, Benin
  • 2Physiology Division, Nigeria Institute for Oil Palm Research, Benin City, Benin
  • 3Agricultural Economics Division, Nigeria Institute for Oil Palm Research, Benin City, Benin
  • 4Department of Plant Biology and Biotechnology, University of Benin, Benin

Res. J. Agriculture & Forestry Sci., Volume 11, Issue (1), Pages 33-39, March,8 (2023)

Abstract

The study was aimed at achieving possible successful in vitro culture of coconut palm (Cocos nucifera L.) Four (4) modified Eeuwensmedia were identified and augmented with various concentrations and combinations of plant growth regulators (auxins and cytokinins). Non- chlorophyllous immature leaves and immature inflorescences of coconut served as explants and were cultured onto the modified media and incubated under dark conditions in the growth room at 25oC. In order to ensure viable cultures, bi- weekly subcultures were carried out for eight (8) weeks. The results showed that calli was formed on the immature inflorescences explants in the modified Eeuwens medium augmented with 2,4-Dichlorophenoxy acetic acid (2,4-D) at 60mg/l in combinations with 2 mg/l6-Benzyl amino purine (BAP) after six (6) weeks of culture. The results obtained showed that calli generation was not possible from both explants at 2,4-D concentrations between 0 - 10 mg/l and otherplant growth regulators used for media supplementation.

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